Yao Chen, Li Gang, Cai Ming, Qian Yeyong, Wang Liqin, Xiao Li, Thaiss Friedrich, Shi Bingyi
Organ Transplant Institute, Beijing, China.
IIII Medizinische Klinik, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany.
Cancer Biomark. 2017 Jul 19;20(1):23-29. doi: 10.3233/CBM-170525.
The goal of our study was to assess the prognostic impact of the necroptosis relative protein RIPK1 genetic polymorphism in ischemia-reperfusion injury and survival after hepatectomy in hepatocellular carcinoma (HCC) patients.
In this study, expression of RIPK1 and its genetic polymorphism(rs2272990) were examined in plasma of 44 HCC patients. All these patients were undergoing partial hepatectomy. The prognostic values of RIPK1 genetic polymorphism for tumor development and survival, and ischemia-reperfusion injury after hepatectomy were further determined.
Plasma RIPK1 expressions were significantly increased in HCC patients, compared to the healthy control group. Totally 19 patients have the GA + AA genotype in the RIPK1 rs2272990 SNP site and 25 have GG genotype. There were no statistically significant intergroup differences observed in age, gender, AFP value, HBV positive, tumor size or cirrhosis. GG genotype had positive correlation with TNM classification (p= 0.033) and lymphatic metastasis (p= 0.027) and was significantly associated with severe hepatic ischemia-reperfusion injury and decreased survival rate after hepatectomy.
In conclusion, the RIPK1 polymorphism is an indicator of hepatic injury and a novel prognostic biomarker for tumor development and survival of HCC recipients after hepatectomy.
本研究的目的是评估坏死性凋亡相关蛋白RIPK1基因多态性对肝细胞癌（HCC）患者肝切除术后缺血再灌注损伤和生存的预后影响。
在本研究中，检测了44例接受部分肝切除术的HCC患者血浆中RIPK1的表达及其基因多态性（rs2272990）。进一步确定了RIPK1基因多态性对肿瘤发生、生存以及肝切除术后缺血再灌注损伤的预后价值。
与健康对照组相比，HCC患者血浆中RIPK1表达显著升高。在RIPK1 rs2272990 SNP位点，共有19例患者具有GA + AA基因型，25例具有GG基因型。在年龄、性别、甲胎蛋白值、乙肝病毒阳性、肿瘤大小或肝硬化方面，未观察到组间有统计学显著差异。GG基因型与TNM分期（p = 0.033）和淋巴转移（p = 0.027）呈正相关，并且与严重的肝缺血再灌注损伤以及肝切除术后生存率降低显著相关。
总之，RIPK1基因多态性是肝损伤的一个指标，也是HCC患者肝切除术后肿瘤发生和生存的一种新的预后生物标志物。
