a Graduate Program in Health Sciences, CBS , Cruzeiro do Sul University , São Paulo , SP , Brazil.
b Graduate Program in Environmental and Experimental Pathology , São Paulo State University , São Paulo , SP , Brazil.
Nutr Neurosci. 2019 Feb;22(2):119-131. doi: 10.1080/1028415X.2017.1356030. Epub 2017 Jul 31.
The purpose of this study was to evaluate some indicators of redox status, and inflammation on different regions of the central nervous system (CNS) of obese rats treated with green tea (GT). We hypothesized that obesity could affect the redox balance in different brain regions due to the diverse nature of the cells as well as the selective neuronal vulnerability to oxidative stress, and GT could triggers benefits effects restoring the redox status.
Male Wistar rats were treated with GT by gavage (12 weeks/5 days/week; 500 mg/kg of body weight) and obesity was induced by cafeteria diet (8 weeks). After this period, the animals were killed and brain tissue (cerebral cortex, cerebellum, and brainstem) was removed to evaluate oxidative stress and inflammation (cytokine release).
We showed that the cafeteria diet had little effect on redox balance in the cerebral cortex and cerebellum; however, the brainstem was the region of the CNS most sensitive to cafeteria diet-induced redox unbalance. GFAP expression was increased in the cerebral cortex of obese rats and reduced by GT. It was also evident that GT treatment had numerous beneficial effects against oxidative damage to biomolecules in all brain regions analyzed.
Our study established that different CNS regions show selective neuronal vulnerability when exposed to a diet enriched with fats and sugars, and the beneficial effect of GT was similar among these regions. We conclude that GT could be a good strategy for improving and maintaining brain function under healthy and pathological conditions.
本研究旨在评估肥胖大鼠经绿茶（GT）处理后中枢神经系统（CNS）不同区域的氧化还原状态和炎症的一些指标。我们假设肥胖可能会由于细胞的多样性以及神经元对氧化应激的选择性易感性而影响不同脑区的氧化还原平衡，而 GT 可能会引发恢复氧化还原状态的有益作用。
雄性 Wistar 大鼠通过灌胃（12 周/5 天/周；500mg/kg 体重）给予 GT 治疗，并通过 cafeteria 饮食（8 周）诱导肥胖。在此期间，处死动物并取出脑组织（大脑皮质、小脑和脑干），以评估氧化应激和炎症（细胞因子释放）。
我们表明 cafeteria 饮食对大脑皮质和小脑的氧化还原平衡几乎没有影响；然而，脑干是对 cafeteria 饮食引起的氧化还原失衡最敏感的 CNS 区域。肥胖大鼠大脑皮质中的 GFAP 表达增加，而 GT 则减少了这种表达。此外，GT 治疗对所有分析的脑区的生物分子氧化损伤具有多种有益作用。
我们的研究表明，不同的 CNS 区域在暴露于富含脂肪和糖的饮食时表现出选择性的神经元易感性，而 GT 的有益作用在这些区域是相似的。我们得出结论，GT 可能是在健康和病理条件下改善和维持大脑功能的一种良好策略。
