Bezerra Maria Luiza Rolim, Gouveia-Nhanca Mirela, da Veiga Dutra Maria Letícia, Batista Kamila Sabino, de Araújo Alana Natalícia Vasconcelos, Dos Santos Lima Marcos, Ribeiro Mateus Duarte, Silva Alexandre Sergio, Alves Adriano Francisco, Pimentel Tatiana Colombo, Magnani Marciane, de Souza Aquino Jailane
Experimental Nutrition Laboratory-LANEX, Department of Nutrition, Federal University of Paraíba (UFPB), João Pessoa, Brazil.
Post Graduate Program in Nutrition Sciences, Federal University of Paraíba (UFPB), João Pessoa, Brazil.
Front Nutr. 2025 Feb 6;12:1524642. doi: 10.3389/fnut.2025.1524642. eCollection 2025.
Obesity is a disease associated with increased oxidative stress in humans and animals, and consumption of antioxidant compounds such as polyphenols can minimise it. These compounds are abundant in malícia ( L.) honey produced by stingless bees. This study aimed to evaluate whether administration of L. honey to obese rats could reduce oxidative stress in vital organs through phenolic compound action.
Wistar rats (228 ± 14.69 g) were randomly divided into two groups: a healthy group (HG, = 20) fed a control diet and an obese group (OG, = 20) fed a cafeteria diet for the initial 8 weeks. After this period, these groups were again randomised into four subgroups: healthy (HG, = 10), obese (OG, = 10), healthy with malícia honey administration (1,000 mg/kg; HGH, = 10), and obese with malícia honey administration (1,000 mg/kg; OGH, = 10) for the final 8 weeks fed the previously mentioned diets. The rats were euthanised at the end of the experiment to collect brain, gut, kidney, and liver tissues to evaluate parameters related to oxidative stress and phenolic profile.
The administration of malícia honey reduced energy intake and weight gain in the OGH in comparison to the OG. Total antioxidant capacity increased in the brain, liver, and gut in both groups treated with honey compared to respective controls. Lipid peroxidation decreased in the brain, gut, and kidney of the OGH. Both treated groups showed elevated phenolic compound deposition, including catechin, procyanidins, and flavonoids, across all organs. Specifically, the brain in the OGH showed greater procyanidin B2 and gallic acid deposition; the liver showed increased procyanidin B1 and B2, epicatechin, and myricetin concentrations; the gut showed higher procyanidin B2 and kaempferol 3-glucoside concentrations; and the kidneys had increased catechin, procyanidin B1 and B2, and gallic acid deposition compared to the OG.
Histologically, the OGH displayed reduced neuronal damage and prevention of hepatic steatosis induced by the cafeteria diet. Malícia honey effectively reduced oxidative stress via modulation of phenolic compounds in the brain, gut, kidney, and liver of cafeteria diet-induced obese rats.
肥胖是一种在人类和动物中与氧化应激增加相关的疾病，食用抗氧化化合物（如多酚）可以将其降至最低。这些化合物在无刺蜂生产的malícia（L.）蜂蜜中含量丰富。本研究旨在评估给肥胖大鼠喂食malícia蜂蜜是否能通过酚类化合物的作用降低重要器官的氧化应激。
将体重为228±14.69g的Wistar大鼠随机分为两组：健康组（HG，n = 20）喂食对照饮食，肥胖组（OG，n = 20）在最初8周喂食自助餐饮食。在此期间过后，这些组再次随机分为四个亚组：健康组（HG，n = 10）、肥胖组（OG，n = 10）、喂食malícia蜂蜜的健康组（1000mg/kg；HGH，n = 10）和喂食malícia蜂蜜的肥胖组（1000mg/kg；OGH，n = 10），在最后8周喂食前述饮食。实验结束时对大鼠实施安乐死，以收集脑、肠道、肾脏和肝脏组织，评估与氧化应激和酚类特征相关的参数。
与OG组相比，给OGH组大鼠喂食malícia蜂蜜可降低能量摄入和体重增加。与各自的对照组相比，两组喂食蜂蜜的大鼠脑、肝脏和肠道中的总抗氧化能力均有所增加。OGH组大鼠脑、肠道和肾脏中的脂质过氧化作用降低。两个处理组在所有器官中的酚类化合物沉积均有所增加，包括儿茶素、原花青素和类黄酮。具体而言，OGH组大鼠脑中的原花青素B2和没食子酸沉积增加；肝脏中表儿茶素、原花青素B1和B2以及杨梅素的浓度增加；肠道中原花青素B2和山奈酚3 - 葡萄糖苷的浓度升高；与OG组相比，肾脏中儿茶素、原花青素B1和B2以及没食子酸的沉积增加。
组织学上，OGH组显示神经元损伤减少，且预防了自助餐饮食诱导的肝脂肪变性。malícia蜂蜜通过调节自助餐饮食诱导的肥胖大鼠脑、肠道、肾脏和肝脏中的酚类化合物，有效降低了氧化应激。
