Balootaki Pariya Ahmadi, Amin Mansour, Haghparasti Farkhondeh, Rokhbakhsh-Zamin Farokh
Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
Department of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman, Iran.
Iran J Med Sci. 2017 Jul;42(4):377-383.
() is generally transmitted into the gastrointestinal tract of animals by the intake of contaminated food or water and causes great economic loss in agriculture worldwide. Some of the spp. are the causative agents of erysipeloid, which is an occupational infection in humans. The aim of the present study was to isolate from animals as well as the hands of the butchers working in Ahvaz, Iran, and to determine their susceptibility to antibiotics.
Totally, 150 samples were taken from slaughterhouse workers, fishermen, and livers and hearts of sheep and calves by the swabbing method. Phenotypical methods and polymerase chain reaction (PCR) were used for the isolation and identification of . The isolates were tested for their susceptibility to commonly used antimicrobial agents using the disk diffusion protocol described by the Clinical and Laboratory Standards Institute.
Out of the 150 samples examined via phenotypical and biochemical tests, 16 samples were positive as putative . twelve cases out of the 16 putative . were confirmed by PCR. The tested isolates were highly sensitive to the antibiotics used. The results of the sensitivity and specificity of PCR revealed that the sensitivity and specificity of indirect PCR were higher than those of direct PCR.
is widely distributed on seafood and presents as a commensal pathogen in nature and animals. Infection with this microorganism should be emphasized because it is a rare organism causing severe infections such as infectious endocarditis and polyarthritis following localized infections.
（）通常通过摄入受污染的食物或水传播到动物的胃肠道，在全球农业中造成巨大经济损失。一些（菌种名称）是类丹毒的病原体，这是一种人类职业感染。本研究的目的是从伊朗阿瓦士的动物以及屠宰工人的手部分离（该菌种），并确定它们对抗生素的敏感性。
通过擦拭法共采集了150份样本，包括屠宰场工人、渔民以及绵羊和小牛的肝脏和心脏。采用表型方法和聚合酶链反应（PCR）进行（该菌种）的分离和鉴定。使用临床和实验室标准协会描述的纸片扩散法检测分离株对常用抗菌剂的敏感性。
通过表型和生化试验检测的150份样本中，有16份样本作为假定的（该菌种）呈阳性。16份假定的（该菌种）样本中有12例通过PCR得到证实。所测试的分离株对所用抗生素高度敏感。PCR敏感性和特异性的结果表明，间接PCR的敏感性和特异性高于直接PCR。
（该菌种）广泛分布于海鲜中，在自然界和动物中表现为共生病原体。应强调感染这种微生物，因为它是一种罕见的病原体，可导致严重感染，如局部感染后发生的感染性心内膜炎和多关节炎。
