Daneshparvar Afrooz, Mowlavi Gholamreza, Mirjalali Hamed, Hajjaran Homa, Mobedi Iraj, Naddaf Saeed Reza, Shidfar Mohammadreza, Sadat Makki Mahsa
Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2017 Apr-Jun;12(2):224-229.
Demodicosis is one of the most prevalent skin diseases resulting from infestation by mites. This parasite usually inhabits in follicular infundibulum or sebaceous duct and transmits through close contact with an infested host.
This study was carried from September 2014 to January 2016 at Tehran University of Medical Sciences, Tehran, Iran. DNA extraction and amplification of 16S ribosomal RNA was performed on four isolates, already obtained from four different patients and identified morphologically though clearing with 10% Potassium hydroxide (KOH) and microscopical examination. Amplified fragments from the isolates were compared with GeneBank database and phylogenetic analysis was carried out using MEGA6 software.
A 390 bp fragment of 16S rDNA was obtained in all isolates and analysis of generated sequences showed high similarity with those submitted to GenBank, previously. Intra-species similarity and distance also showed 99.983% and 0.017, respectively, for the studied isolates. Multiple alignments of the isolates showed Single Nucleotide Polymorphisms (SNPs) in 16S rRNA fragment. Phylogenetic analysis revealed that all 4 isolates clustered with other recovered from GenBank database. Our accession numbers KF875587 and KF875589 showed more similarity together in comparison with two other studied isolates.
Mitochondrial 16S rDNA is one of the most suitable molecular barcodes for identification and this fragment can use for intra-species characterization of the most human-infected mites.
蠕形螨病是由螨虫感染引起的最常见皮肤病之一。这种寄生虫通常栖息在毛囊漏斗部或皮脂腺导管中，并通过与受感染宿主的密切接触传播。
本研究于2014年9月至2016年1月在伊朗德黑兰医科大学进行。对从四名不同患者身上已获得的四个分离株进行16S核糖体RNA的DNA提取和扩增，并通过用10%氢氧化钾（KOH）透明处理和显微镜检查进行形态学鉴定。将分离株的扩增片段与基因库数据库进行比较，并使用MEGA6软件进行系统发育分析。
所有分离株均获得了一个390bp的16S rDNA片段，对所产生序列的分析表明与先前提交到GenBank的序列具有高度相似性。所研究分离株的种内相似性和距离分别为99.983%和0.017。分离株的多重比对显示16S rRNA片段存在单核苷酸多态性（SNP）。系统发育分析表明，所有4个分离株与从基因库数据库中回收的其他分离株聚类。我们的登录号KF875587和KF875589相比其他两个研究分离株彼此间显示出更高的相似性。
线粒体16S rDNA是最适合用于鉴定的分子条形码之一，该片段可用于大多数人类感染螨虫的种内特征分析。
