基于线粒体16S rDNA的螨类分子鉴定及系统发育关系
Molecular identification and phylogenetic relationship of mites based on mitochondrial 16S rDNA.
作者信息
Prasher Pawan, Baghra Dolly, Singh Drishtant, Thakur Sharad, Gill Navpreet Kaur, Kesavan Anup Kumar
机构信息
Sri Guru Ram Das Institute of Medical Science and Research, Amritsar, Punjab, India.
Department of Zoology and Environmental Sciences, Punjabi University, Patiala, Punjab, India.
出版信息
Trop Parasitol. 2020 Jul-Dec;10(2):136-141. doi: 10.4103/tp.TP_76_19. Epub 2021 Jan 25.
BACKGROUND AND OBJECTIVE
mites are tiny parasites that live around hair follicles of mammals. The two main species of i.e. and brevis present in humans are found near the hair follicles of eyes. The present study was to understand the presence of mites in people suffering from blepharitis in Amritsar, Punjab.
MATERIAL AND METHODS
mites samples present in blepharitis patients were isolated from the eyelashes. DNA was isolated from three mites and used for PCR amplification of mitochondrial (mt) 16S rDNA. The amplified PCR product were purified and used for molecular identification.
RESULTS
The amplified mt16s rDNA product was sequenced and subjected to BLAST search in the NCBI database for molecular identification. The identified mite belongs to folliculorum species. The phylogenetic tree constructed by using mt16s rDNA sequence suggests that is closer to than to .
CONCLUSION
All the three isolates belong to and the mitochondrial DNA 16S rDNA partial sequence is applicable for phylogenetic relationship analysis.
背景与目的
螨虫是生活在哺乳动物毛囊周围的微小寄生虫。人类眼部存在的两种主要螨虫，即毛囊蠕形螨和皮脂蠕形螨，在眼周毛囊附近被发现。本研究旨在了解旁遮普邦阿姆利则睑缘炎患者中螨虫的存在情况。
材料与方法
从睑缘炎患者的睫毛中分离出螨虫样本。从三只螨虫中提取DNA，并用于线粒体（mt）16S rDNA的PCR扩增。扩增后的PCR产物经纯化后用于分子鉴定。
结果
对扩增后的mt16s rDNA产物进行测序，并在NCBI数据库中进行BLAST搜索以进行分子鉴定。鉴定出的螨虫属于毛囊蠕形螨物种。利用mt16s rDNA序列构建的系统发育树表明，毛囊蠕形螨与皮脂蠕形螨相比，与某种螨的亲缘关系更近。
结论
所有三个分离株均属于毛囊蠕形螨，线粒体DNA 16S rDNA部分序列适用于系统发育关系分析。
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