Assunção Paulo Isaac Dias, da Conceição Edemilson Cardoso, Borges Leonardo Luiz, de Paula Joelma Abadia Marciano
Campus Anápolis de Ciências Exatas e Tecnológicas, Universidade Estadual de Goiás, Caixa Postal 459, 75132-903 Anápolis, GO, Brazil.
Laboratório de Pesquisa, Desenvolvimento & Inovação de Bioprodutos, Universidade Federal de Goiás, Caixa Postal 131, 74605-170 Goiânia, GO, Brazil.
Evid Based Complement Alternat Med. 2017;2017:1501038. doi: 10.1155/2017/1501038. Epub 2017 Jul 6.
A simple HPLC-UV method has been developed and validated for the quantification of ellagic acid (EA) in ethanol extracts of L. (Myrtaceae) leaves. The ultrasound-assisted extraction (UAE) optimization was performed using a Box Behnken design (3) combined with response surface methodology to study the effects of the ethanol concentration (%, w/w), extraction time (minutes), and temperature (°C) on the EA concentration. The optimized results showed that the highest extraction yield of EA by UAE was 26.0 g mL when using 44% (w/w) ethanol as the solvent, 22 minutes as the extraction time, and 59°C as the extraction temperature. The concentration of EA in relation to the predicted value was 93.7% ± 0.4. UAE showed a strong potential for EA extraction.
已开发并验证了一种简单的高效液相色谱 - 紫外检测法，用于定量测定桃金娘科番樱桃属植物叶片乙醇提取物中的鞣花酸（EA）。采用Box - Behnken设计（3因素）结合响应面法对超声辅助提取（UAE）进行优化，以研究乙醇浓度（%，w/w）、提取时间（分钟）和温度（°C）对EA浓度的影响。优化结果表明，当使用44%（w/w）乙醇作为溶剂、提取时间为22分钟、提取温度为59°C时，UAE对EA的最高提取率为26.0 μg/mL。EA的浓度与预测值的关系为93.7% ± 0.4。UAE在EA提取方面显示出强大的潜力。
