Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, De ogyeong-daero 1732, Giheung-gu, Yongin 17104, Korea.
Department of Integrated Biomedical and Life Science, Korea University, Seoul 02841, Korea.
Molecules. 2021 Jan 7;26(2):267. doi: 10.3390/molecules26020267.
Toward the standardization of common evening primrose ( sprout extract (OBS-E), we aimed to obtain indicator compounds and use a validated method. HPLC-UVD allowed simultaneous quantification of the indicator compounds quercetin-3--glucuronide and ellagic acid. The method was validated in terms of specificity, linearity, precision, accuracy, and limit of detection/limit of quantification (LOD/LOQ). High specificity and linearity was demonstrated, with correlation coefficients of 1.0000 for quercetin-3--glucuronide and 0.9998 for ellagic acid. The LOD/LOQ values were 0.486/1.472 μg/mL for quercetin-3--glucuronide and 1.003/3.039 μg/mL for ellagic acid. Intra-day and inter-day variability tests produced relative standard deviation for each compound of <2%, a generally accepted precision criterion. High recovery rate were also obtained, indicating accuracy validation. The OBS-E prepared using various concentrations of ethanol were then analyzed. The 50% ethanol extract had highest content of quercetin-3--glucuronide, whereas the 70% ethanol extract possessed the lowest. However, the ellagic acid content was highest in the 70% ethanol extract and lowest in the 90% ethanol extract. Thus, quercetin-3--glucuronide and ellagic acid can be used industrially as indicator compounds for sprout products, and our validated method can be used to establish indicator compounds for other natural products.
为了实现普通月见草（发芽提取物（OBS-E）的标准化，我们旨在获得指标化合物并使用经过验证的方法。HPLC-UVD 允许同时定量测定指示化合物槲皮素-3-O-葡萄糖醛酸苷和鞣花酸。该方法在特异性、线性、精密度、准确性和检测限/定量限（LOD/LOQ）方面进行了验证。证明具有高度的特异性和线性，槲皮素-3-O-葡萄糖醛酸苷的相关系数为 1.0000，鞣花酸的相关系数为 0.9998。槲皮素-3-O-葡萄糖醛酸苷和鞣花酸的 LOD/LOQ 值分别为 0.486/1.472μg/mL 和 1.003/3.039μg/mL。日内和日间变异性测试产生的每个化合物的相对标准偏差均<2%，这是一个普遍接受的精密度标准。还获得了高回收率，表明准确性得到了验证。然后分析了使用不同浓度乙醇制备的 OBS-E。50%乙醇提取物中槲皮素-3-O-葡萄糖醛酸苷含量最高，而 70%乙醇提取物中含量最低。然而，70%乙醇提取物中鞣花酸含量最高，90%乙醇提取物中含量最低。因此，槲皮素-3-O-葡萄糖醛酸苷和鞣花酸可以作为工业上芽产品的指标化合物，我们验证的方法可以用于建立其他天然产物的指标化合物。
