Liu Wenrong, Ding Ruofan, Zhang Yiming, Mao Canquan, Kang Ran, Meng Junhua, Huang Qingqing, Xiong Lili, Guo Zhiyun
School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
Biotechnol Lett. 2017 Nov;39(11):1639-1647. doi: 10.1007/s10529-017-2407-1. Epub 2017 Jul 31.
To characterize the transcriptome profile of hepatocellular carcinoma (HCC) HepG2 cells treated with peptide 9R-P201 for further functional verification and HCC drug development.
1557 mRNAs (1125 upregulated and 432 downregulated) and 881 lncRNAs (640 upregulated and 241 downregulated) with significant differential expression were identified using RNA-seq. The qRT-PCR results showed that the differential expression of several mRNAs and lncRNAs coincided with the RNA-seq results. Differentially expressed mRNAs and lncRNAs presented a significant difference in genomic characteristics but no preference under 9R-P201 treatment compared with control. The GO and KEGG functional enrichment analyses showed that differentially expressed mRNAs and lncRNAs remarkably enriched in cancer-related biological processes and signaling pathways. Finally, we screened out 33 TFs, 273 lncRNAs and 94 target genes with high degree interaction which were remarkably associated with the tumorigenesis and progression of cancers using betweenness centrality analysis.
These findings offer novel insights into the mechanism of 9R-P201 in HepG2 cells and provide new opportunities for the future 9R-P201-based drug development and the treatment of hepatocellular carcinoma.
对用肽9R-P201处理的肝癌HepG2细胞的转录组图谱进行表征，以进行进一步的功能验证和肝癌药物开发。
使用RNA测序鉴定出1557个mRNA（1125个上调和432个下调）和881个lncRNA（640个上调和241个下调）具有显著差异表达。qRT-PCR结果表明，几种mRNA和lncRNA的差异表达与RNA测序结果一致。与对照相比，差异表达的mRNA和lncRNA在基因组特征上呈现出显著差异，但在9R-P201处理下没有偏好。GO和KEGG功能富集分析表明，差异表达的mRNA和lncRNA显著富集于癌症相关的生物学过程和信号通路。最后，我们使用中间中心性分析筛选出33个转录因子、273个lncRNA和94个具有高度相互作用的靶基因，它们与癌症的发生和进展显著相关。
这些发现为9R-P201在HepG2细胞中的作用机制提供了新的见解，并为未来基于9R-P201的药物开发和肝癌治疗提供了新的机会。
