Gong Xianli, Wei Wei, Chen Lan, Xia Zhi, Yu Chengbo
Department of Radiation Oncology, The First Affiliated Hospital, College of Medicine Zhejiang University, Hangzhou 310003, China.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine Zhejiang University, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
Oncotarget. 2016 Jul 5;7(27):42422-42430. doi: 10.18632/oncotarget.9880.
Hepatocellular carcinoma (HCC) is one of the most common kinds of malignancies and is closely correlated with hepatitis B virus (HBV) infection. Recent evidence has proved that long non-coding RNAs (lncRNAs) are implicated in development and progression of cancer. However, the contributions of lncRNAs to HBV-related HCC remain largely unknown. Here, we comprehensively investigated lncRNA expression profiles in HBV-related HCC by annotating and analyzing microarray datasets. By analyzing 42 HCC tissue samples with different etiology (HBV-related, alcohol-related, and primary HCC) and 15 normal liver tissues, we identified 182 lncRNAs that were specifically differentially expressed in HBV-related HCC, namely HBV-related HCC specific lncRNAs(HH-lncRNAs). Using an online function annotation tool, we found these HH-lncRNAs were associated many oncogenes and immunity related biological processes. 6 candidate HH-lncRNAs were selected and further validated by quantitative real-time PCR analysis in a cohort of HCC tissue samples. Function of a candidate HH-lncRNAs, BAIAP2-AS1, was further predicted by co-expression network and gene set enrichment analysis. These findings provide insights into HH-lncRNAs and offer resource for further search of biomarkers and therapeutic targets of HBV-related HCC.
肝细胞癌（HCC）是最常见的恶性肿瘤之一，与乙型肝炎病毒（HBV）感染密切相关。最近的证据表明，长链非编码RNA（lncRNAs）与癌症的发生和发展有关。然而，lncRNAs在HBV相关HCC中的作用仍 largely未知。在这里，我们通过注释和分析微阵列数据集，全面研究了HBV相关HCC中的lncRNA表达谱。通过分析42个具有不同病因（HBV相关、酒精相关和原发性HCC）的HCC组织样本和15个正常肝组织，我们鉴定出182个在HBV相关HCC中特异性差异表达的lncRNAs，即HBV相关HCC特异性lncRNAs（HH-lncRNAs）。使用在线功能注释工具，我们发现这些HH-lncRNAs与许多癌基因和免疫相关的生物学过程有关。选择了6个候选HH-lncRNAs，并在一组HCC组织样本中通过定量实时PCR分析进一步验证。通过共表达网络和基因集富集分析进一步预测了一个候选HH-lncRNAs，BAIAP2-AS1的功能。这些发现为HH-lncRNAs提供了见解，并为进一步寻找HBV相关HCC的生物标志物和治疗靶点提供了资源。
