Bierer Barbara E, Strober Warren
Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland.
Curr Protoc Immunol. 2017 Aug 1;118:A.3F.1-A.3F.5. doi: 10.1002/cpim.27.
Peripheral blood is the chief source of mononuclear cells (lymphocytes and monocytes) for immunologic studies of humans. This appendix presents protocols for obtaining blood by simple venipuncture when relatively small amounts of blood (10 to 100 ml) are necessary or by lymphapheresis when large amounts (300 to 5000 ml) are necessary. Cells collected by these procedures can be further separated by techniques described in Chapter 7. © 2017 by John Wiley & Sons, Inc.
外周血是用于人类免疫学研究的单核细胞（淋巴细胞和单核细胞）的主要来源。本附录介绍了在需要相对少量血液（10至100毫升）时通过简单静脉穿刺采血的方案，以及在需要大量血液（300至5000毫升）时通过淋巴细胞分离术采血的方案。通过这些程序收集的细胞可以用第7章中描述的技术进一步分离。© 2017约翰威立国际出版公司。
