Feige U, Sorg C
J Immunol Methods. 1984 Jan 20;66(1):161-70. doi: 10.1016/0022-1759(84)90258-8.
Continuous flow centrifugation leukapheresis allows the continuous removal of leukocytes from the peripheral blood of individual donors. With the IBM 2997 cell separator, leukapheresis of granulocytes has been well established. On the other hand, no standard procedure for the collection of lymphocytes and/or monocytes has yet been described. Here, we investigated the conditions necessary for collection of large amounts of mononuclear cells with highest possible purity. The average yield from more than 100 healthy donors was 8 X 10(9) leukocytes per donor containing 80-100% mononuclear cells (MNC). Of the MNC, 10-55% were monocytes and 45-90% lymphocytes, depending on the donor. The rotor speed for maximal MNC collection was between 800 and 1000 rpm. Highest yields of monocytes were obtained at 900 rpm. No sedimenting agents were added to the blood. With more than 150 donors no rebound leukocytosis or lasting depression of blood cell counts was observed after leukapheresis. One patient with the Sézary syndrome who was subjected to leukapheresis for 26 months at 3-6 week intervals showed no pathological changes in his blood cell count to date. The disease was stabilized and no abnormalities in resistance to infection became apparent.
连续流动离心白细胞单采术可连续从个体供血者的外周血中去除白细胞。使用IBM 2997细胞分离器，粒细胞单采术已得到充分确立。另一方面，尚未描述淋巴细胞和/或单核细胞采集的标准程序。在此，我们研究了收集大量纯度尽可能高的单核细胞所需的条件。100多名健康供血者的平均产量为每位供血者8×10⁹个白细胞，其中含80 - 100%的单核细胞（MNC）。在MNC中，10 - 55%为单核细胞，45 - 90%为淋巴细胞，这取决于供血者。收集最大量MNC的转子速度在800至1000转/分钟之间。在900转/分钟时获得最高产量的单核细胞。血液中未添加沉降剂。超过150名供血者在白细胞单采术后未观察到白细胞增多反弹或血细胞计数持续下降。一名患有Sezary综合征的患者每隔3 - 6周进行白细胞单采术26个月，迄今为止其血细胞计数未出现病理变化。疾病得到稳定，未出现抗感染能力异常。
