Iwamaru Yoshifumi, Mathiason Candace K, Telling Glenn C, Hoover Edward A
a Prion Research Center, Department of Microbiology, Immunology and Pathology , College of Veterinary Medicine and Biomedical Sciences, Colorado State University , Fort Collins , CO , USA.
b Prion Disease Research Unit , National Institute of Animal Health , Tsukuba , Ibaraki , Japan.
Prion. 2017 Jul 4;11(4):277-283. doi: 10.1080/19336896.2017.1336273. Epub 2017 Aug 1.
A possible strategy to develop more diverse cell culture systems permissive to infection with naturally occurring prions is to exploit culture of neurospheres from transgenic mice expressing the normal prion protein (PrP) of the native host species. Accordingly, we developed differentiated neurosphere cultures from the cervid PrP-expressing mice to investigate whether this in vitro system would support replication of non-adapted cervid-origin chronic wasting disease (CWD) prions. Here we report the successful amplification of disease-associated PrP in differentiated neurosphere cultures within 3 weeks after exposure to CWD prions from both white-tailed deer or elk. This neurosphere culture system provides a new in vitro tool that can be used to assess non-adapted CWD prion propagation and transmission.
开发更多样化的允许天然存在的朊病毒感染的细胞培养系统的一种可能策略是利用来自表达天然宿主物种正常朊病毒蛋白（PrP）的转基因小鼠的神经球培养物。因此，我们从表达鹿PrP的小鼠中开发了分化的神经球培养物，以研究这种体外系统是否支持非适应性鹿源慢性消耗病（CWD）朊病毒的复制。在此，我们报告在暴露于来自白尾鹿或麋鹿的CWD朊病毒后3周内，分化的神经球培养物中成功扩增了与疾病相关的PrP。这种神经球培养系统提供了一种新的体外工具，可用于评估非适应性CWD朊病毒的传播和传染。
