Zhang Liwei, Li Guangyu, Shi Meng, Liu Hsin-Hua, Ge Shaokui, Ou Yvonne, Flanagan John G, Chen Lu
Center for Eye Disease and Development, Vision Science Graduate Program, University of California, Berkeley, California, United States 2School of Optometry and Vision Science, University of California, Berkeley, California, United States 3Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha, China.
Center for Eye Disease and Development, Vision Science Graduate Program, University of California, Berkeley, California, United States 2School of Optometry and Vision Science, University of California, Berkeley, California, United States.
Invest Ophthalmol Vis Sci. 2017 Aug 1;58(10):3879-3886. doi: 10.1167/iovs.16-20807.
This study was designed to develop and characterize a laser-induced model of acute intraocular hypertension that permits the study of the anterior segment of the eye.
CD1 mice aged 5 and 8 weeks were examined for elevation of IOP induced by laser photocoagulation. We compared between occlusion of episcleral veins alone and when combined with 270° limbal vessel occlusion. Anterior chamber angle, corneal thickness, and retinal nerve fiber layer (RNFL) thickness were evaluated by anterior- and posterior-segment optical coherence tomography (OCT). Additionally, at day 7 post-procedure, the anterior segment was evaluated for inflammatory cellular presentation by histologic analysis and OCT, and limbal vessels and whole-mount retina were immunostained for CD31 and Brn3a, respectively. Brn3a-positive retinal ganglion cells (RGCs) were quantified with ImageJ software.
After single or combined laser treatment in mice aged 5 or 8 weeks, IOP was significantly elevated for 5 to 6 days before returning to the baseline by day 7 post-procedure. Anterior segment assessment indicated less synechiae in the anterior chamber angle and better preserved limbal vessels with single versus combined laser treatment. Corneal thickness was significantly increased after single or combined treatment. No inflammatory cells were detected in the anterior chamber. The thickness of the RNFL and the density of RGCs were both significantly reduced after single or combined treatment.
Laser photocoagulation of episcleral veins alone in CD1 mice aged 5 to 8 weeks may be used to induce ocular hypertension resulting in RNFL thinning and ganglion cell loss. This model permits the study of the anterior as well as the posterior segment of the eye.
本研究旨在建立并表征一种激光诱导的急性眼内高压模型，以用于眼前节的研究。
对5周龄和8周龄的CD1小鼠进行激光光凝诱导眼压升高的检测。我们比较了单纯巩膜静脉阻塞以及与270°角膜缘血管阻塞联合使用时的情况。通过前后节光学相干断层扫描（OCT）评估前房角、角膜厚度和视网膜神经纤维层（RNFL）厚度。此外，在术后第7天，通过组织学分析和OCT评估眼前节的炎性细胞表现，分别对角膜缘血管和全层视网膜进行CD31和Brn3a免疫染色。使用ImageJ软件对Brn3a阳性视网膜神经节细胞（RGCs）进行定量分析。
在5周龄或8周龄小鼠进行单次或联合激光治疗后，眼压在术后第7天恢复到基线水平之前显著升高5至6天。眼前节评估表明，与联合激光治疗相比，单次激光治疗时前房角粘连较少，角膜缘血管保存较好。单次或联合治疗后角膜厚度显著增加。前房未检测到炎性细胞。单次或联合治疗后RNFL厚度和RGCs密度均显著降低。
对5至8周龄的CD1小鼠单纯进行巩膜静脉激光光凝可用于诱导眼压升高，导致RNFL变薄和神经节细胞丢失。该模型可用于眼前节和眼后节的研究。
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