鉴定和测定硒同型半胱氨酸——啤酒酵母中主要的硒化合物。
Identification and determination of selenohomolanthionine - The major selenium compound in Torula yeast.
机构信息
CNRS/UPPA, Institute for Analytical Sciences and Physical Chemistry for the Environment and Materials (IPREM), UMR 5254, Hélioparc, F-64053 Pau, France.
Laboratory of Toxicology and Environmental Health, Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8675, Japan.
出版信息
Food Chem. 2017 Dec 15;237:1196-1201. doi: 10.1016/j.foodchem.2017.06.042. Epub 2017 Jun 8.
Torula yeast (Candida utilis) was found to metabolize selenium in a totally different way to Brewer's yeast (S. cerevisiae) leading to the biosynthesis of selenohomolanthionine (SeHLan), a major selenium compound accounting for 60-80% of the total selenium. The identity of SeHLan was confirmed by retention time matching in hydrophilic ion interaction chromatography (HILIC) with inductively coupled plasma mass spectrometric detection (ICP MS) using a custom synthesized standard molecule and by HILIC - Orbitrap MS and MS-MS fragmentation. Selenohomolanthionine escapes the current assays for the organic character of Se-rich yeast based on the protein-bound selenomethionine determination. A HILIC - ICP MS method was developed for the quantitative determination of selenohomolanthionine in yeast supplements with a detection limit of 146ng/g.
圆酵母（Candida utilis）被发现以与酿酒酵母（Saccharomyces cerevisiae）完全不同的方式代谢硒，从而合成了主要的硒化合物硒同型高半胱氨酸（SeHLan），占总硒的 60-80%。通过使用定制合成的标准分子在亲水相互作用色谱（HILIC）中的保留时间匹配，并通过 HILIC-Orbitrap MS 和 MS-MS 碎片分析，用电感耦合等离子体质谱（ICP-MS）检测，确认了 SeHLan 的身份。硒同型高半胱氨酸逃避了当前基于蛋白质结合硒代蛋氨酸测定的富硒酵母有机特性的检测方法。开发了一种用于酵母补充剂中硒同型高半胱氨酸定量测定的 HILIC-ICP-MS 方法，检测限为 146ng/g。
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