一种用于从猪组织中选择性纯化3-甲基喹喔啉-2-羧酸的免疫亲和柱及其通过带紫外检测的高效液相色谱法和基于胶体金的免疫色谱分析法进行的测定。
An immunoaffinity column for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid from swine tissues and its determination by high-performance liquid chromatography with ultraviolet detection and a colloidal gold-based immunochromatographic assay.
作者信息
Peng Dapeng, Zhang Xiya, Wang Yulian, Pan Yuanhu, Liu Zhenli, Chen Dongmei, Sheng Feng, Yuan Zonghui
机构信息
National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
出版信息
Food Chem. 2017 Dec 15;237:290-296. doi: 10.1016/j.foodchem.2017.05.115. Epub 2017 May 23.
An immunoaffinity column (IAC) for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) from porcine muscle and the liver as well as the methods for its determination by high-performance liquid chromatography with ultraviolet detection (HPLC-UV) and a colloidal gold-based immunochromatographic assay (GICA) were developed. This is the first study that describes a clean-up method that employs an IAC for the determination of MQCA. Using the HPLC-UV method, the limits of detection and limits of quantification for MQCA in different samples were 1.0-3.0μgkg and 4.0-10.0μgkg, respectively. The average recoveries of MQCA that was spiked into samples at concentrations of 4.0-100μgkg were 80.1-87.7%, with relative standard deviations of less than 8.5%. Using the GICA method, the limits of detection for MQCA in different samples were 10μgkg and 50μgkg. No false negatives or false positives were observed.
开发了一种用于从猪肌肉和肝脏中选择性纯化3-甲基喹喔啉-2-羧酸（MQCA）的免疫亲和柱（IAC），以及采用紫外检测高效液相色谱法（HPLC-UV）和胶体金免疫层析法（GICA）对其进行测定的方法。这是第一项描述采用IAC净化方法测定MQCA的研究。使用HPLC-UV方法，不同样品中MQCA的检测限和定量限分别为1.0-3.0μg/kg和4.0-10.0μg/kg。以4.0-100μg/kg的浓度添加到样品中的MQCA的平均回收率为80.1-87.7%，相对标准偏差小于8.5%。使用GICA方法，不同样品中MQCA的检测限为10μg/kg和50μg/kg。未观察到假阴性或假阳性。
Zotero 插件