Zhang Yaqing, Zhou Song, Chang Xiaoxi, Tao Xiaoqi
College of Food Science, Southwest University.
Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, Southwest University.
Anal Sci. 2019 Dec 10;35(12):1291-1293. doi: 10.2116/analsci.19C009. Epub 2019 Nov 22.
We have reported a sensitive chemiluminescent competitive indirect enzyme-linked immunosorbent assay (CL-ciELISA) based on immunomagnetic beads separation, purification and enrichment for the simultaneous determination of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) and quinoxaline-2-carboxylic acid (QCA) in edible animal tissues. Forty field samples were analyzed with the developed CL-ciELISA, and the results correlated well with those obtained in liquid chromatography-tandem mass spectrometry (LC-MS/MS), confirming the utility of CL-ciELISA for the quantitation of MQCA and QCA in fish, shrimp, pork and chicken with a good accuracy.
我们报道了一种基于免疫磁珠分离、纯化和富集的灵敏化学发光竞争性间接酶联免疫吸附测定法（CL-ciELISA），用于同时测定食用动物组织中的3-甲基喹喔啉-2-羧酸（MQCA）和喹喔啉-2-羧酸（QCA）。用所建立的CL-ciELISA分析了40个现场样品，结果与液相色谱-串联质谱法（LC-MS/MS）获得的结果高度相关，证实了CL-ciELISA在准确测定鱼、虾、猪肉和鸡肉中MQCA和QCA含量方面的实用性。
