College of Food Science, Southwest University.
Anal Sci. 2020 Jul 10;36(7):783-785. doi: 10.2116/analsci.20C008. Epub 2020 Jun 19.
A novel rapid method based on a background fluorescence quenching immunochromatographic assay (bFQICA) was established to achieve simultaneously the quantitative detection of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) and quinoxaline-2-carboxylic acid (QCA), which were efficiently extracted and enriched 4 times using immunomagnetic beads from pork. The analysis of field pork samples by bFQICA was in accordance with that of LC-MS/MS; especially, the proposed bFQICA exhibited great advantages in convenience and efficiency, which only takes 30 min for the detection of MQCA and QCA.
建立了一种基于背景荧光猝灭免疫层析分析（bFQICA）的新型快速方法，可同时实现从猪肉中高效提取和富集 4 倍的 3-甲基喹喔啉-2-羧酸（MQCA）和喹喔啉-2-羧酸（QCA）的定量检测。bFQICA 对现场猪肉样品的分析结果与 LC-MS/MS 一致；特别是，所提出的 bFQICA 在方便性和效率方面具有很大的优势，仅需 30 分钟即可检测 MQCA 和 QCA。
