Lopez-Oceja A, Nuñez C, Baeta M, Gamarra D, de Pancorbo M M
BIOMICS Research Group, University of the Basque Country, UPV/EHU, Vitoria-Gasteiz 01006, Spain.
BIOMICS Research Group, University of the Basque Country, UPV/EHU, Vitoria-Gasteiz 01006, Spain.
Food Chem. 2017 Dec 15;237:701-706. doi: 10.1016/j.foodchem.2017.06.004. Epub 2017 Jun 3.
Meat adulteration by substitution with lower value products and/or mislabeling involves economic, health, quality and socio-religious issues. Therefore, identification and traceability of meat species has become an important subject to detect possible fraudulent practices. In the present study the development of a high resolution melt (HRM) screening method for the identification of eight common meat species is reported. Samples from Bos taurus, Ovis aries, Sus scrofa domestica, Equus caballus, Oryctolagus cuniculus, Gallus gallus domesticus, Meleagris gallopavo and Coturnix coturnix were analyzed through the amplification of a 148 bp fragment from the cyt b gene with a universal primer pair in HRM analyses. Melting profiles from each species, as well as from several DNA mixtures of these species and blind samples, allowed a successful species differentiation. The results demonstrated that the HRM method here proposed is a fast, reliable, and low-cost screening technique.
用低价值产品替代和/或错误标注来掺假肉类涉及经济、健康、质量和社会宗教问题。因此，肉类物种的鉴定和可追溯性已成为检测可能欺诈行为的重要课题。在本研究中，报告了一种用于鉴定八种常见肉类物种的高分辨率熔解（HRM）筛选方法的开发。通过在HRM分析中使用通用引物对扩增细胞色素b基因的148 bp片段，对来自牛、绵羊、家猪、马、家兔、家鸡、火鸡和鹌鹑的样本进行了分析。每个物种以及这些物种的几种DNA混合物和盲样的熔解曲线实现了成功的物种区分。结果表明，本文提出的HRM方法是一种快速、可靠且低成本的筛选技术。
