Preckel Laura, Brünen-Nieweler Claudia, Denay Grégoire, Petersen Henning, Cichna-Markl Margit, Dobrovolny Stefanie, Hochegger Rupert
Chemical and Veterinary Analytical Institute Muensterland-Emscher-Lippe (CVUA-MEL), Joseph-Koenig-Strasse 40, 48147 Muenster, Germany.
Chemical and Veterinary Analytical Institute Rhein-Ruhr-Wupper (CVUA-RRW), Deutscher Ring 100, 47798 Krefeld, Germany.
Foods. 2021 Nov 20;10(11):2875. doi: 10.3390/foods10112875.
The substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing role in food authentication. In the present study, we investigated the applicability of a DNA metabarcoding method for routine analysis of mammalian and poultry species in food and pet food products. We analyzed a total of 104 samples (25 reference samples, 56 food products and 23 pet food products) by DNA metabarcoding and by using a commercial DNA array and/or by real-time PCR. The qualitative and quantitative results obtained by the DNA metabarcoding method were in line with those obtained by PCR. Results from the independent analysis of a subset of seven reference samples in two laboratories demonstrate the robustness and reproducibility of the DNA metabarcoding method. DNA metabarcoding is particularly suitable for detecting unexpected species ignored by targeted methods such as real-time PCR and can also be an attractive alternative with respect to the expenses as indicated by current data from the cost accounting of the AGES laboratory. Our results for the commercial samples show that in addition to food products, DNA metabarcoding is particularly applicable to pet food products, which frequently contain multiple animal species and are also highly prone to adulteration as indicated by the high portion of analyzed pet food products containing undeclared species.
用商业价值较低的动物物种替代更受青睐的动物物种是肉类产品掺假的常见类型。DNA宏条形码技术，即DNA条形码技术与下一代测序（NGS）的结合，在食品鉴定中发挥着越来越重要的作用。在本研究中，我们调查了一种DNA宏条形码技术方法在食品和宠物食品中哺乳动物和禽类物种常规分析中的适用性。我们通过DNA宏条形码技术，并使用商业DNA芯片和/或实时荧光定量PCR对总共104个样品（25个参考样品、56个食品和23个宠物食品）进行了分析。DNA宏条形码技术方法获得的定性和定量结果与PCR获得的结果一致。在两个实验室对七个参考样品子集进行的独立分析结果证明了DNA宏条形码技术方法的稳健性和可重复性。DNA宏条形码技术特别适用于检测诸如实时荧光定量PCR等靶向方法忽略的意外物种，并且就AGES实验室成本核算的当前数据所示的费用而言，它也是一种有吸引力的替代方法。我们对商业样品的结果表明，除食品外，DNA宏条形码技术特别适用于宠物食品，如分析的宠物食品中含有未申报物种的比例很高所示，宠物食品经常含有多种动物物种，也极易掺假。
