Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.
Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan; Learning Park, King Mongkut's University of Technology Thonburi (Ratchaburi), Rangbua, Chombung, Ratchaburi 70150, Thailand.
Food Chem. 2017 Dec 15;237:749-755. doi: 10.1016/j.foodchem.2017.05.145. Epub 2017 Jun 10.
The effect of electrostatic atomized water particle (EAWP) treatment on quality maintenance during postharvest storage was determined to clarify a physiological role of reactive oxygen species in broccoli florets. The EAWP treatment suppressed the floret yellowing of broccoli during storage. The hydrogen peroxide content increased immediately after EAWP treatment but then remained at a low level during storage. Catalase (CAT) activity increased rapidly with EAWP treatment and was higher than that in the control for the first 4days of storage. The BoCAT gene expression level with EAWP treatment also showed a high trend as compared to the control. The gene expressions of BoRboh, BoCu/ZnSOD, and BoAPX showed almost the same tendency with or without EAWP treatment. These findings suggested that hydrogen peroxide formed by EAWP treatment and regulated by CAT could be relevant to maintaining the quality of broccoli.
为了阐明活性氧在青花菜小花中的生理作用,确定了静电雾化水颗粒(EAWP)处理对采后贮藏期间品质维持的影响。EAWP 处理抑制了青花菜贮藏期间的小花黄化。过氧化氢含量在 EAWP 处理后立即增加,但在贮藏过程中保持在低水平。过氧化氢酶(CAT)活性随着 EAWP 处理迅速增加,在前 4 天的贮藏中高于对照。与对照相比,EAWP 处理的 BoCAT 基因表达水平也呈上升趋势。BoRboh、BoCu/ZnSOD 和 BoAPX 的基因表达无论是否进行 EAWP 处理都呈现出几乎相同的趋势。这些发现表明,由 EAWP 处理形成并由 CAT 调节的过氧化氢可能与维持青花菜的品质有关。