Department of Horticultural Science, Imam Khomeini International University, Qazvin 34148-96818, Iran.
Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan 51664, Iran.
Food Chem. 2020 Dec 15;333:127481. doi: 10.1016/j.foodchem.2020.127481. Epub 2020 Jul 8.
In this study, we tested the exogenous application of phytosulfokine α (PSKα) for delaying the yellowing of broccoli florets during cold storage. Our results showed that the lower yellowing in broccoli florets treated with 150 nM PSKα was probably due to the higher endogenous accumulation of PSKα, leading to the endogenous accumulation of guanosine 3', 5'-cyclic monophosphate (cGMP). Besides, broccoli florets treated with 150 nM PSKα exhibited a higher accumulation of phenols and flavonoids by triggering gene expression and activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). Moreover, the higher expression of L-galactotno-1,4-lactone dehydrogenase (GLDH) gene and the lower expression of ascorbic acid oxidase (AAO) gene in broccoli florets treated with 150 nM PSKα may be the reasons for the higher accumulation of ascorbic acid. In conclusion, the exogenous application of PSKα is a promising strategy in delaying the yellowing and preserving the nutritional quality of broccoli florets during cold storage.
在这项研究中，我们测试了外源施用植物磺基丙氨酸（PSKα）对延缓低温贮藏过程中青花菜变黄的作用。结果表明，用 150 nM PSKα 处理的青花菜较低的黄化程度可能是由于内源 PSKα 的积累较高，导致鸟苷 3'，5'-环单磷酸（cGMP）的内源性积累。此外，用 150 nM PSKα 处理的青花菜通过触发苯丙氨酸解氨酶（PAL）和查尔酮合酶（CHS）的基因表达和活性，表现出酚类和类黄酮的更高积累。此外，用 150 nM PSKα 处理的青花菜中 L-半乳糖酸-1,4-内酯脱氢酶（GLDH）基因的更高表达和抗坏血酸氧化酶（AAO）基因的更低表达可能是抗坏血酸积累更高的原因。总之，外源施用 PSKα是一种有前途的策略，可以延缓青花菜在冷藏过程中的黄化和保持其营养品质。
