College of Food Science and Technology, Jilin University, Changchun 130062, PR China.
Key Laboratory for Molecular Enzymology & Engineering of the Ministry of Education, School of Life Science, Jilin University, Changchun, PR China.
Food Chem. 2017 Dec 15;237:793-802. doi: 10.1016/j.foodchem.2017.05.144. Epub 2017 May 30.
Effects of pulse electric field (PEF) on antioxidant activity of pine nut (Pinus koraiensis) peptide were discussed using HO-induced HepG2 cells and changes of peptide structures were measured by MIR, NMR and CD spectra. Using HPLC-MS/MS, a novel peptide was identified as QDHCH. After PEF treatment the DPPH and ABTS radical inhibition, and CAA values of QDHCH were increased to 85.13%±0.17%, 95.45%±0.12%, and 4670.10μmol of quercetin equivalents/100g. The PEF-treated QDHCH has better protective oxidative stress inhibitory of 74.22±3.70%, and the T-SOD, CAT, GSH-Px and GSH-Rx activities in cells were significantly increased by 91.92, 7.98, 18.5 and 18.79U/mg prot, while the MDA content was decreased to 8.45±0.71U/mg prot compared with HO damaged group. In addition, the hydroxyl radical scavenging activity of QDHCH was increased by 10.53%; the basic structure was not changed by PEF, while the influenced secondary structures may induce the antioxidant activity improvement in HepG2 cells.
采用 HO 诱导 HepG2 细胞模型，探讨了脉冲电场（PEF）对松子肽抗氧化活性的影响，并通过 MIR、NMR 和 CD 光谱测量了肽结构的变化。采用 HPLC-MS/MS，鉴定出一种新型肽为 QDHCH。经 PEF 处理后，QDHCH 的 DPPH 和 ABTS 自由基抑制率和 CAA 值分别提高到 85.13%±0.17%、95.45%±0.12%和 4670.10μmol 槲皮素当量/100g。PEF 处理的 QDHCH 对氧化应激抑制的保护作用更好，抑制率为 74.22±3.70%，细胞中 T-SOD、CAT、GSH-Px 和 GSH-Rx 活性分别显著提高了 91.92、7.98、18.5 和 18.79U/mg prot，而 MDA 含量降低至 8.45±0.71U/mg prot，与 HO 损伤组相比。此外，QDHCH 的羟基自由基清除活性提高了 10.53%；PEF 并未改变其基本结构，但影响的二级结构可能会诱导 HepG2 细胞中抗氧化活性的提高。
