Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Science, East China Normal University, 500 Dong Chuan Road, Shanghai 200241, PR China.
Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Science, East China Normal University, 500 Dong Chuan Road, Shanghai 200241, PR China.
Bioresour Technol. 2017 Nov;243:1149-1158. doi: 10.1016/j.biortech.2017.07.077. Epub 2017 Jul 16.
In this work, anammox-denitrification process was verified by N stable isotopic tracing methods and variations and effects of microbial community structures were studied using Illumina MiSeq sequencing and Quantitative Polymerase Chain Reaction (qPCR). The results showed that higher nitrogen removal efficiency and richer microbial consortia was observed at hydraulic loading rate (HLR) of 15L/m·d, BOD/TN ratio of 0.4:1, respectively. Proteobacteria, Chloroflexi, Acidobacteria and Firmicutes were the dominant phyla in the anamox-denitrification biomass. The number of amx gene changed significantly during the HLR downshift and BOD/TN ratio upshift period. The obtained results enhance understanding regarding the microbial community structures of anammox-denitrification bacteria in aged refuse, leading to a more effective controlling of anammox-denitrification process.
本工作采用氮稳定同位素示踪方法验证了厌氧氨氧化-反硝化过程，利用 Illumina MiSeq 测序和定量聚合酶链反应 (qPCR) 研究了微生物群落结构的变化和影响。结果表明，水力停留时间为 15L/m·d、BOD/TN 比为 0.4:1 时，氮去除效率更高，微生物群落更丰富。在厌氧氨氧化-反硝化生物量中，变形菌门、绿弯菌门、酸杆菌门和厚壁菌门是主要的门类。在水力停留时间降低和 BOD/TN 比升高的过程中，amx 基因的数量发生了显著变化。研究结果增强了对老龄垃圾中厌氧氨氧化-反硝化菌微生物群落结构的理解，有助于更有效地控制厌氧氨氧化-反硝化过程。
