Gore Rucha Diwakar, Palaskar Sangeeta Jayant, Bartake Anirudha Ratnadeep
Postgraduate Student, Department of Oral Pathology and Microbiology, Sinhgad Dental College and Hospital, Pune, Maharashtra, India.
Professor and Head, Department of Oral Pathology and Microbiology, Sinhgad Dental College and Hospital, Pune, Maharashtra, India.
J Clin Diagn Res. 2017 Jun;11(6):ZC40-ZC42. doi: 10.7860/JCDR/2017/26316.10057. Epub 2017 Jun 1.
Oral squamous cell carcinoma is the sixth most common cancer worldwide and is one of the leading causes of death especially in developing countries like India. It is usually managed with the help of chemotherapeutic agents that are toxic not only to the tumor cells but also to normal cells in many ways. Whereas the traditional herbal medicines which are used from ancient days in India are generally known to be free of toxic effects and are effective. Wheatgrass is the young grass of the wheat plant '', family Poaceae (Gramineae). It contains a number of vitamins, mineral, amino acids and vital enzymes, these play an important role in the anticancer approach of this herbal product. It is also called as 'green blood' because of its high content of chlorophyll, which bears a structural similarity to haemoglobin and this result in high oxygen supply to all the body tissues.
To analyse the effect of the aqueous wheat grass extract on Oral Squamous Cell Carcinoma (OSCC) cell line by MTT assay.
'KB cell line' (Mouth Epidermal Carcinoma Cells) was used for the present study. Aqueous extract of wheat grass was prepared in the institution. The aim of the study was to assess the effect of the aqueous wheat grass extract on KB cell line(OSCC) by MTT assay.
A 41.4% of OSCC cell inhibition was observed at 1000 µg/ml dilution of aqueous wheatgrass extract in 24 hours.
The aqueous extract of wheatgrass has an inhibitory effect on the oral cancer cell line proliferation.
口腔鳞状细胞癌是全球第六大常见癌症,是主要的死亡原因之一,尤其是在印度等发展中国家。它通常借助化疗药物进行治疗,这些药物不仅对肿瘤细胞有毒性,而且在许多方面对正常细胞也有毒性。而印度自古以来使用的传统草药通常已知无毒且有效。小麦草是禾本科(Gramineae)小麦植物的嫩草。它含有多种维生素、矿物质、氨基酸和重要酶类,这些在这种草药产品的抗癌方法中发挥着重要作用。由于其叶绿素含量高,它也被称为“绿色血液”,叶绿素与血红蛋白在结构上相似,这导致向身体所有组织提供高氧供应。
通过MTT法分析小麦草水提取物对口腔鳞状细胞癌(OSCC)细胞系的作用。
本研究使用“KB细胞系”(口腔表皮癌细胞)。小麦草水提取物在本机构制备。本研究的目的是通过MTT法评估小麦草水提取物对KB细胞系(OSCC)的作用。
在24小时内,在1000μg/ml稀释的小麦草水提取物中观察到41.4%的OSCC细胞抑制率。
小麦草水提取物对口腔癌细胞系增殖具有抑制作用。