Pauleti Nathielli Nayara, Mello Jonas, Siebert Diogo Alexandre, Micke Gustavo Amadeu, de Albuquerque Cláudia Almeida Coelho, Alberton Michele Debiasi, Barauna Sara Cristiane
a Departamento de Ciências Naturais , Universidade Regional de Blumenau (FURB) , Blumenau , Brazil.
b Departamento de Química , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , Brazil.
Nat Prod Res. 2018 Aug;32(16):1987-1990. doi: 10.1080/14786419.2017.1359167. Epub 2017 Aug 1.
This study evaluates the antidepressant-like effect and analysed the qualitative and quantitative 74 phenolic standards of ethyl acetate fraction from Tabernaemontana catharinensis leaves. Acute administration of fraction in mice reduced the immobility time in forced swimming and tail suspension tests confirming its antidepressant-like activity. The anti-immobility effect elicited by this fraction was prevented by the pretreatment of mice with PCPA (100 mg kg), ketanserin (5 mg kg), SCH 23,390 (0.05 mg kg) or yohimbine (1 mg kg). A sub effective dose of the fraction produced a synergistic effect with fluoxetine (5 mg kg). Chromatographic analysis identified 4-hydroxybenzoic and p-coumaric acids in the ethyl acetate fraction from T. catharinensis. Capillary electrophoresis presented 7.34 ± 0.02 mg g of p-coumaric acid concentration in the fraction. Therefore, it is possible that antidepressant-like effect elicited by ethyl acetate fraction from T. catharinensis be dependent on the p-coumaric acid.
本研究评估了卡塔林山橙叶乙酸乙酯部位的抗抑郁样作用，并分析了其74种酚类成分的定性和定量情况。对小鼠急性给予该部位提取物可减少强迫游泳和悬尾试验中的不动时间，证实其具有抗抑郁样活性。用对氯苯丙氨酸（100 mg/kg）、酮色林（5 mg/kg）、SCH 23,390（0.05 mg/kg）或育亨宾（1 mg/kg）预处理小鼠可阻断该部位提取物引起的抗不动作用。该部位提取物的亚有效剂量与氟西汀（5 mg/kg）产生协同作用。色谱分析鉴定出卡塔林山橙叶乙酸乙酯部位含有4-羟基苯甲酸和对香豆酸。毛细管电泳显示该部位中对香豆酸的浓度为7.34±0.02 mg/g。因此，卡塔林山橙叶乙酸乙酯部位引起的抗抑郁样作用可能依赖于对香豆酸。
