Programa de Pós-Graduação em Saúde e Meio Ambiente, Universidade da Região de Joinville- UNIVILLE, Rua Paulo Malschitzki,10- Zona Industrial Norte, CEP 89201-972, Joinville, SC, Brazil.
Departamento de Ciências Naturais, Centro de Ciências Exatas e Naturais, Universidade Regional de Blumenau, Rua Antônio daVeiga, 140, CEP 89030-203, Blumenau, SC, Brazil.
Metab Brain Dis. 2018 Dec;33(6):1985-1994. doi: 10.1007/s11011-018-0306-3. Epub 2018 Aug 22.
This work investigated the antioxidant and antidepressant-like effects of ethyl acetate extract from Eugenia catharinensis in mice treated with corticosterone (20 mg/Kg). The animals received saline or corticosterone (21 days) and, in the last 7 days, they were treated with the extract (50, 125, 200 or 250 mg/Kg) or vehicle. After 24 h, the mice were submitted to the open field and forced swimming tests, after which the hippocampus and cerebral cortex were removed. Our results showed that the extract decreased the immobility time of mice in the forced swimming test and that the extract was able to reverse the effect caused by corticosterone. Corticosterone pre-treatment generated oxidative stress, altering antioxidant enzymes in the nervous tissue. The extract increased the catalase and superoxide dismutase activities and reversed the effects of corticosterone. In the hippocampus, the extract increased superoxide dismutase activity and reversed the increase in catalase activity elicited by corticosterone. We propose that the effects elicited by the Eugenia catharinensis are dependent on the presence of phenolic compounds (gallic acid, protocatechuic acid, syringic acid, 4-hydroxy methylbenzoic acid, chlorogenic acid, salicylic acid, caffeic acid, vanillic acid, p-coumaric acid, isoquercetin, rutin, ferulic acid, aromadendrin, galangin and apigenin) in this extract, as demonstrated by HPLC-ESI-MS/MS.
本研究旨在探讨丁香酸乙酯提取物对皮质酮（20mg/kg）处理小鼠的抗氧化和抗抑郁样作用。动物接受生理盐水或皮质酮（21 天），并在最后 7 天接受提取物（50、125、200 或 250mg/kg）或载体处理。24h 后，将小鼠置于旷场和强迫游泳试验中，然后取出海马和大脑皮质。我们的结果表明，提取物可减少强迫游泳试验中小鼠的不动时间，且提取物可逆转皮质酮引起的效应。皮质酮预处理产生氧化应激，改变神经组织中的抗氧化酶。提取物增加了过氧化氢酶和超氧化物歧化酶的活性，并逆转了皮质酮的作用。在海马中，提取物增加了超氧化物歧化酶的活性，并逆转了皮质酮引起的过氧化氢酶活性增加。我们提出，丁香酸乙酯的作用依赖于该提取物中存在的酚类化合物（没食子酸、原儿茶酸、丁香酸、4-羟甲基苯甲酸、绿原酸、水杨酸、咖啡酸、香草酸、对香豆酸、异槲皮苷、芦丁、阿魏酸、橙皮苷、杨梅素和芹菜素），这一点通过 HPLC-ESI-MS/MS 得到了证实。
