College of Medical Laboratory, Dalian Medical University, Dalian, 116044, China.
J Transl Med. 2017 Aug 1;15(1):166. doi: 10.1186/s12967-017-1267-8.
This study aimed to establish a model that estimates the survival advantage at the molecular level based on telomere length and serum biomarkers of aging, to explore clinical significance.
The study consisted of 100 healthy subjects and 40 type 2 diabetes mellitus patients, 20-90 years of age. Saliva telomere relative length (LnTL) was measured by the quantitative real-time polymerase chain reaction and the serum biochemical parameters, including albumin (ALB), total proteins, total cholesterol, triglycerides, and some enzyme parameters were detected by a biochemical analyzer. The Z values were transformed from mean values and standard deviations to estimate the survival advantage. A normal reference range (95% confidence interval) was set to the comprehensive advantage of the Z values (Z) to evaluate the comprehensive survival advantage.
The Z values of serum ALB and saliva LnTL could be used to estimate the survival advantage, and effectively distinguish between the aging and nonaging individuals. The Z was greater than 1.64 in the normal reference range, and type 2 diabetes mellitus patients had lower survival advantages compared to those of the control group (p < 0.05).
Our two-dimensional model system using ALB and LnTL was valid and may have potential applications for evaluating the aging status at the molecular level, and for the observation of disease characteristics.
本研究旨在建立一种基于端粒长度和血清衰老生物标志物的分子水平生存优势的预测模型，探讨其临床意义。
本研究纳入 100 名健康受试者和 40 名 2 型糖尿病患者，年龄 20-90 岁。采用实时荧光定量聚合酶链反应检测唾液端粒相对长度（LnTL），采用生化分析仪检测血清生化参数，包括白蛋白（ALB）、总蛋白、总胆固醇、甘油三酯和部分酶学参数。将平均值和标准差转化为 Z 值，以估计生存优势。将 Z 值的综合优势（Z）设定为正态参考范围（95%置信区间），以评估综合生存优势。
血清 ALB 和唾液 LnTL 的 Z 值可用于估计生存优势，并能有效区分衰老和非衰老个体。Z 值在正常参考范围内大于 1.64，2 型糖尿病患者的生存优势低于对照组（p<0.05）。
本研究采用 ALB 和 LnTL 的二维模型系统有效，可以潜在地用于评估分子水平的衰老状态，以及观察疾病特征。
Zotero 插件
