Cho Hyejoung, Kim Okjoon, Lee Younghee, Kang Li-Jung, Nguyen Cam Ngoc, Ishihara Atsushi, Kim Hye-Eun
Department of Oral Pathology, Dental Science Research Institute and Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea.
Department of Pharmacology, Ajou University School of Medicine, Suwon 16499, Republic of Korea.
Biochem Biophys Res Commun. 2017 Sep 30;491(4):973-979. doi: 10.1016/j.bbrc.2017.07.158. Epub 2017 Jul 29.
Feruloylserotonin (FS) is a major bioactive component of safflower seeds, with documented strong antibacterial, anti-inflammatory, and free radical scavenging activities. Reactive oxygen species (ROS) can strongly induce melanogenesis and cell apoptosis. The present study aimed to investigate the ability of FS in preventing hydrogen peroxide (HO)-induced melanogenesis and cell apoptosis. Melanogenesis and apoptotic cell death were induced by transient exposure to HO in B16F10 and SK-Mel-2 melanoma cells. FS significantly inhibited melanogenesis and cell death in both cell lines. FS inhibited HO-induced melanin production by down-regulating CREB/MITF/TYR signaling via inhibited intracellular cAMP accumulation. Additionally, FS induced extracellular regulated kinase activation, which led to the degradation of MITF and consequently decreased TYR expression and melanin production in HO-stimulated cells. Furthermore, FS inhibited HO-induced apoptotic cell death by maintaining mitochondrial membrane potential. Therefore, FS might have potential use for cosmetic whitening and as a therapeutic agent for hyperpigmentation disorder.
阿魏酰基血清素（FS）是红花籽的一种主要生物活性成分，有文献记载其具有强大的抗菌、抗炎和自由基清除活性。活性氧（ROS）能强烈诱导黑色素生成和细胞凋亡。本研究旨在探究FS预防过氧化氢（HO）诱导的黑色素生成和细胞凋亡的能力。通过短暂暴露于HO诱导B16F10和SK-Mel-2黑色素瘤细胞发生黑色素生成和凋亡性细胞死亡。FS在两种细胞系中均显著抑制黑色素生成和细胞死亡。FS通过抑制细胞内cAMP积累，下调CREB/MITF/TYR信号传导，从而抑制HO诱导的黑色素生成。此外，FS诱导细胞外调节激酶激活，导致MITF降解，进而降低HO刺激细胞中TYR的表达和黑色素生成。此外，FS通过维持线粒体膜电位抑制HO诱导的凋亡性细胞死亡。因此，FS可能具有用于化妆品美白以及作为色素沉着紊乱治疗剂的潜在用途。
