Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3.
Swiss Federal Institute for Forest, Snow and Landscape Research, Birmensdorf CH-8903, Switzerland.
Sci Data. 2017 Jul 25;4:170092. doi: 10.1038/sdata.2017.92. eCollection 2017.
The scarcity of long-term data on soil microbial communities in the decades following timber harvesting limits current understanding of the ecological problems associated with maintaining the productivity of managed forests. The high complexity of soil communities and the heterogeneity of forest and soil necessitates a comprehensive approach to understand the role of microbial processes in managed forest ecosystems. Here, we describe a curated collection of well replicated, multi-faceted data from eighteen reforested sites in six different North American ecozones within the Long-term Soil Productivity (LTSP) Study, without detailed analysis of results or discussion. The experiments were designed to contrast microbial community composition and function among forest soils from harvested treatment plots with varying intensities of organic matter removal. The collection includes 724 bacterial (16S) and 658 fungal (ITS2) amplicon libraries, 133 shotgun metagenomic libraries as well as stable isotope probing amplicon libraries capturing the effects of harvesting on hemicellulolytic and cellulolytic populations. This collection serves as a foundation for the LTSP Study and other studies of the ecology of forest soil and forest disturbance.
在采伐后几十年内，土壤微生物群落的长期数据稀缺，限制了当前对维持管理森林生产力相关生态问题的理解。土壤群落的高度复杂性和森林及土壤的异质性需要综合的方法来理解微生物过程在管理森林生态系统中的作用。在这里，我们描述了一个经过精心整理的、多方面的数据集，来自 LTSP 研究中的六个不同北美的生态区的 18 个重新造林点，其中不包括对结果的详细分析或讨论。这些实验旨在对比来自不同有机物质去除强度采伐处理区的森林土壤中的微生物群落组成和功能。该数据集包括 724 个细菌（16S）和 658 个真菌（ITS2）扩增子文库、133 个 shotgun 宏基因组文库以及稳定同位素探测扩增子文库，这些文库捕捉了采伐对半纤维素分解菌和纤维素分解菌种群的影响。该数据集为 LTSP 研究以及其他森林土壤和森林干扰生态学研究提供了基础。
