Ferreira do Nascimento Nivaldo, de Siqueira-Silva Diógenes Henrique, Pereira-Santos Matheus, Fujimoto Takafumi, Senhorini José Augusto, Nakaghi Laura Satiko Okada, Yasui George Shigueki
Aquaculture Center,São Paulo State University,Via de Acesso Prof. Paulo Donato Castellane s/n,Jaboticabal,SP 14884-900,Brazil.
Laboratory of Fish Biotechnology,National Center for Research and Conservation of Continental Fish,Chico Mendes Institute of Biodiversity Conservation,Rodovia Pref. Euberto Nemésio Pereira de Godoy,Pirassununga,SP 13630-970,Brazil.
Zygote. 2017 Aug;25(4):537-544. doi: 10.1017/S0967199417000399. Epub 2017 Aug 2.
This study aimed to examine the gonadal morphology of diploid and triploid fish through stereological analysis. Triploid individuals were obtained after temperature shock (40°C for 2 min) at 2 min post-fertilization and reared until 175 days post-fertilization (dpf). Intact eggs were used to obtain the diploids. Gonads were collected for histological analysis at 83, 114, 144 and 175 dpf. Diploid females and males presented normal oogenesis and spermatogenesis through all the experimental period. Conversely, stereological analysis revealed that triploid females were sterile and oogonia were the prevalent cell type in the ovaries. Triploid males presented increased amounts of spermatocyte cysts and a large area of lumen when compared with diploids and in addition the amount of spermatozoa was lower than that observed for diploids. However, some triploid males presented spermatogenesis similar to diploids. Therefore, we concluded that triploidization is an interesting alternative to produce sterile individuals in A. altiparanae.
本研究旨在通过体视学分析来检测二倍体和三倍体鱼类的性腺形态。三倍体个体是在受精后2分钟进行温度休克（40°C，持续2分钟）后获得的，并饲养至受精后175天（dpf）。完整的卵用于获得二倍体。在83、114、144和175 dpf时收集性腺用于组织学分析。在整个实验期间，二倍体雌性和雄性呈现正常的卵子发生和精子发生。相反，体视学分析表明三倍体雌性不育，卵原细胞是卵巢中占主导的细胞类型。与二倍体相比，三倍体雄性的精母细胞囊肿数量增加且管腔面积较大，此外精子数量低于二倍体。然而，一些三倍体雄性呈现出与二倍体相似的精子发生。因此，我们得出结论，三倍体化是在高身副脂鲤中产生不育个体的一种有趣的替代方法。
