Department of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 5 Oczapowskiego Str., 10-718 Olsztyn, Poland.
Department of Ecology and Vertebrate Zoology, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha Str., 90-237 Lodz, Poland.
Anim Reprod Sci. 2022 Sep;244:107053. doi: 10.1016/j.anireprosci.2022.107053. Epub 2022 Aug 11.
The changes in concentrations of 17ß-estradiol (E), testosterone (T), 11-ketotestosterone (11-KT) in the gonads and plasma of diploid and triploid gibel carp (Carassius gibelio) from the Siemianowka Reservoir, the Vistula River drainage were quantified and compared using an enzyme immunoassay. The phase of gonad maturity was based on histological analysis and the gonadosomatic index. All fish had properly developed gonads in the pre-spawning, spawning and late spawning phases of the reproductive cycle in the fish collected in April, June and October, respectively. Diploid and triploid females and males did not differ in mean GSI in all reproductive phases. In October, ovaries of most females contained vitellogenic oocytes, testes had large numbers of spermatozoa. The hormone concentrations in both tissues depended mainly on the reproductive phase, but not on ploidy. The patterns of changes in E concentrations was similar in females and males. In the pre-spawning phase, diploid and triploid females showed differences in the concentrations of 11-KT in gonads and plasma, and T in plasma. Diploid males differed in pattern of T concentrations in gonads and plasma, while all males showed a similar pattern of 11-KT plasma concentrations. Spermatozoa observed in triploid males suggest that they participate in reproduction. Gibel carps, regardless of ploidy, had an extended period of reproduction, which makes this invasive species a potentially greater threat to native ichthyofauna. The sex androgen concentrations that differed between 2 n and 3 n females could be physiological factors potentially contributing to the coexistence of gynogenetic C. gibelio females and sexual diploids.
采用酶联免疫分析法，对来自 Siemianowka 水库、维斯瓦河支流的二倍体和三倍体银鲫（Carassius gibelio）性腺和血浆中 17β-雌二醇（E）、睾酮（T）、11-酮睾酮（11-KT）浓度的变化进行了定量分析和比较。性腺成熟阶段基于组织学分析和性腺体指数（GSI）。在分别于 4 月、6 月和 10 月采集的鱼类中，所有鱼类在繁殖周期的产卵前、产卵中和产卵后期都具有发育良好的性腺。在所有生殖阶段，二倍体和三倍体雌鱼和雄鱼的平均 GSI 没有差异。在 10 月，大多数雌鱼的卵巢中含有卵黄生成卵母细胞，睾丸中含有大量精子。两种组织中的激素浓度主要取决于生殖阶段，而与ploidy 无关。E 浓度的变化模式在雌鱼和雄鱼中相似。在产卵前期，二倍体和三倍体雌鱼的性腺和血浆中 11-KT 浓度以及血浆中 T 浓度存在差异。二倍体雄鱼的性腺和血浆中 T 浓度模式存在差异，而所有雄鱼的血浆 11-KT 浓度模式相似。在三倍体雄鱼中观察到的精子表明它们参与了繁殖。银鲫无论ploidy 如何，都有一个延长的繁殖期，这使得这种入侵物种对本地鱼类群构成了更大的潜在威胁。在 2n 和 3n 雌鱼之间存在差异的雄性性类固醇浓度可能是导致银鲫雌核生殖雌性和性二倍体共存的生理因素。
