一种使用迭代磷酸化策略的 Gram 规模 HPLC 免费 TNA 三磷酸合成方法。
A Gram-Scale HPLC-Free Synthesis of TNA Triphosphates Using an Iterative Phosphorylation Strategy.
机构信息
Departments of Pharmaceutical Sciences, Chemistry, and Molecular Biology and Biochemistry, University of California , Irvine, California 92697-3958, United States.
出版信息
Org Lett. 2017 Aug 18;19(16):4379-4382. doi: 10.1021/acs.orglett.7b02099. Epub 2017 Aug 2.
α-l-Threofuranosyl nucleoside 3'-triphosphates (tNTPs) bearing the four genetic bases adenine (A), cytosine (C), guanine (G), and thymine (T) were synthesized on a gram scale using an iterative phosphorylation strategy that avoids the need for tedious HPLC purification. This new synthetic procedure greatly increases the scale on which tNTP substrates can be produced for polymerase-mediated TNA synthesis studies.
α-L-呋喃核糖核苷 3′-三磷酸酯（tNTPs）带有腺嘌呤（A）、胞嘧啶（C）、鸟嘌呤（G）和胸腺嘧啶（T）四种遗传碱基，采用迭代磷酸化策略在克级规模上合成，避免了繁琐的 HPLC 纯化。这种新的合成方法大大提高了 tNTP 底物的生产规模，可用于聚合酶介导的 TNA 合成研究。
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