一锅法合成α-L-苏阿糖呋喃糖基核苷三磷酸（tNTPs）。

A one-pot synthesis of α-l-threofuranosyl nucleoside triphosphates (tNTPs).

作者信息

Sau Sujay P, Chaput John C

机构信息

Department of Pharmaceutical Sciences, University of California, Irvine, CA 92697-3958, USA.

出版信息

Bioorg Med Chem Lett. 2016 Jul 15;26(14):3271-3273. doi: 10.1016/j.bmcl.2016.05.057. Epub 2016 May 20.

DOI:10.1016/j.bmcl.2016.05.057

PMID:27246616

Abstract

TNA (α-l-threofuranosyl nucleoside) triphosphates of adenosine (tATP), guanosine (tGTP), cytidine (tCTP), and thymidine (tTTP) were synthesized from their corresponding 3'-O-phosphoramidite derivatives using a novel one-pot reaction that is less moisture sensitive than traditional methods. The chemically synthesized tNTPs, despite containing an unnatural 3'-triphosphate moiety, are similar in thermal stability to natural nucleotide triphosphates.

摘要

腺苷（tATP）、鸟苷（tGTP）、胞苷（tCTP）和胸苷（tTTP）的TNA（α-L-苏呋喃糖核苷）三磷酸是由其相应的3'-O-磷酰胺衍生物通过一种新型的一锅法反应合成的，该方法比传统方法对水分更不敏感。化学合成的tNTPs尽管含有非天然的3'-三磷酸部分，但其热稳定性与天然核苷酸三磷酸相似。