Monroy R L, MacVittie T J, Darden J H, Schwartz G N, Patchen M L
Exp Hematol. 1986 Nov;14(10):904-11.
Two heterogeneous cell populations (CP 1-7 and CP 8-10) were separated from rhesus monkey bone marrow using counterflow centrifugation-elutriation (CCE). These two cell populations were distinct with respect to morphological composition, expression of cell surface antigens, hemopoietic progenitor cell activity, and concentration of hemopoietic stem cells (HSC). The hemopoietic progenitor cell activity and HSC were concentrated in CP 8-10. In autologous transplantation studies, CP 8-10 reconstituted the lymphohemopoietic system of lethally irradiated monkeys in a manner similar to that of monkeys transplanted with unfractionated bone marrow cells. CP 1-7 was lymphocyte rich and depleted of progenitor cell activity. Transplantation of CP 1-7 led to eventual lymphohemopoietic reconstitution of irradiated monkeys; however, complete engraftment was delayed by as much as 14 days compared to either the transplantation of CP 8-10 or to unfractionated bone marrow. Thus, a presence of the HSC in the lymphocyte-rich progenitor-cell-depleted population can be detected in the rhesus model.
使用逆流离心淘析法（CCE）从恒河猴骨髓中分离出两个异质细胞群（CP 1 - 7和CP 8 - 10）。这两个细胞群在形态组成、细胞表面抗原表达、造血祖细胞活性以及造血干细胞（HSC）浓度方面存在差异。造血祖细胞活性和HSC集中在CP 8 - 10中。在自体移植研究中，CP 8 - 10以类似于用未分级骨髓细胞移植的猴子的方式重建了接受致死性照射猴子的淋巴造血系统。CP 1 - 7富含淋巴细胞且缺乏祖细胞活性。CP 1 - 7的移植最终导致了受照射猴子的淋巴造血重建；然而，与CP 8 - 10移植或未分级骨髓移植相比，完全植入延迟了多达14天。因此，在恒河猴模型中可以检测到富含淋巴细胞且缺乏祖细胞的群体中存在HSC。
