Knospe W H, Husseini S G
Exp Hematol. 1986 Nov;14(10):975-80.
Cellulose ester membranes (CEM) were coated with stromal cells from murine bone or bone marrow irradiated in vitro with 1000, 2000, or 4000 rad and then implanted i.p. in CAF1 mice for periods of six and 12 months. CEM coated with stromal cells from bone showed excellent regeneration of bone and hematopoiesis after 1000 rad in vitro irradiation. After 2000 rad, hematopoietic and bone regeneration was reduced by about 50%, and after 4000 rad it was completely absent in CEM coated with stromal cells from bone. CEM coated with stromal cells from bone marrow showed no regeneration of hematopoiesis or bone after 1000, 2000, and 4000 rad in vitro irradiation and residence i.p. for six and 12 months. These results indicate that regeneration of the hematopoietic microenvironment is dependent upon living stromal cells. A difference in radiation sensitivity is demonstrated between stromal cells from bone and from bone marrow.
将纤维素酯膜（CEM）用经1000、2000或4000拉德体外照射的小鼠骨骼或骨髓中的基质细胞包被，然后腹腔内植入CAF1小鼠体内6个月和12个月。用骨骼基质细胞包被的CEM在1000拉德体外照射后显示出良好的骨再生和造血功能。2000拉德照射后，造血和骨再生减少约50%，4000拉德照射后，用骨骼基质细胞包被的CEM完全没有造血和骨再生。用骨髓基质细胞包被的CEM在1000、2000和4000拉德体外照射并腹腔内植入6个月和12个月后，没有造血或骨再生。这些结果表明造血微环境的再生依赖于活的基质细胞。骨骼和骨髓来源的基质细胞之间显示出辐射敏感性的差异。
