Knospe W H, Husseini S, Trobaugh F E
Exp Hematol. 1978 Aug;6(7):601-12.
Cellulose ester membranes (CEM) were folded into a trilaminar open-ended tube which was implanted into the peritoneal cavity of mice. CEM rapidly acquired a stromal core with many features of marrow such as fat, fibroblasts, an abundant sinusoidal microcirculation and monocyte-macrophage-like cells. CEM took up 59iron, 99technetium sulfur colloid and produced CSF in in vitro culture but their microenvironment supported only granulopoiesis. CEM were coated on their interior surfaces with bone marrow or regenerating medullary cavity mesenchyme or bone but the stromal cores supported only granulopoiesis after 3 weeks to 3 months of implantation. CEM coated with spleen and implanted into mice developed trilineal hematopoiesis within 6 weeks with abundant erythropoiesis and megakaryocytopoiesis in addition to granulopoiesis. These CEM differed from splenic tissue in that only scattered lymphoid tissue was present. CEM coated with bone marrow and bone developed trilineal hematopoiesis but only after3--6 months of peritoneal implantation. CEM coated with regenerating medullary cavity mesenchyme failed to develop trilineal hematopoiesis. Cyclophosphamide injection did not enhance hematopoiesis. These experiments indicate that splenic, marrow and bone tissue contain stromal elements capable of being transferred onto CEM which then develop a microenvironment capable of supporting trilineal hematopoiesis.
将纤维素酯膜（CEM）折叠成三层开口管，植入小鼠腹腔。CEM迅速获得一个具有许多骨髓特征的基质核心，如脂肪、成纤维细胞、丰富的窦状微循环和单核细胞 - 巨噬细胞样细胞。CEM在体外培养中摄取59铁、99锝硫胶体并产生脑脊液，但其微环境仅支持粒细胞生成。CEM的内表面用骨髓、再生髓腔间充质或骨进行包被，但植入3周后至3个月后，基质核心仅支持粒细胞生成。用脾脏包被并植入小鼠的CEM在6周内发展为三系造血，除粒细胞生成外，还有丰富的红细胞生成和巨核细胞生成。这些CEM与脾组织的不同之处在于仅存在散在的淋巴组织。用骨髓和骨包被的CEM在腹腔植入3 - 6个月后才发展为三系造血。用再生髓腔间充质包被的CEM未能发展为三系造血。注射环磷酰胺并未增强造血。这些实验表明，脾、骨髓和骨组织含有能够转移到CEM上的基质成分，然后形成能够支持三系造血的微环境。
