Krogh Nielsen Marie, Hector Sven Magnus, Allen Kelly, Subhi Yousif, Sørensen Torben Lykke
Department of Ophthalmology, Zealand University Hospital, Vestermarksvej 23, DK-4000 Roskilde, Denmark.
Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Immun Ageing. 2017 Jul 27;14:18. doi: 10.1186/s12979-017-0100-9. eCollection 2017.
Neutrophil dysfunction plays a key role in the development of diseases characterized by inflammation and angiogenesis. Here, we studied the systemic expression of neutrophil markers reflecting activation, adhesion, and resolution of inflammation in patients with neovascular age-related macular degeneration (AMD).
This was a prospective case-control study of patients with neovascular AMD and age-matched healthy control individuals. Patients were recruited from an outpatient program, and control individuals were recruited amongst patients' relatives. Current smokers and individuals with either active immune-disease or ongoing cancer were not included, as these factors are known to affect neutrophil function. Fresh-drawn venous blood was processed for flow cytometric analysis of neutrophil markers. We determined percentages of positive cells and compared expression levels using fluorescence intensity measures. We found conditional differences on marker expression between patients with neovascular AMD ( = 29) and controls ( = 28): no differences were found when looking broadly, but several differences emerged when focusing on non-smokers. Here, patients with neovascular AMD had increased expression of the activity marker cluster of differentiation (CD) 66b ( = 0.003; Mann-Whitney U test), decreased expression of adhesion marker CD162 ( = 0.044; Mann-Whitney U test), and lower expression of the resolution of inflammation marker C-X-C chemokine receptor 2 ( = 0.044; Mann-Whitney U test).
We present novel evidence suggesting that the activity of circulating neutrophils, sensitive to smoking, may differ in patients with neovascular AMD.
中性粒细胞功能障碍在以炎症和血管生成特征的疾病发展中起关键作用。在此，我们研究了反映新生血管性年龄相关性黄斑变性（AMD）患者炎症激活、黏附和消退的中性粒细胞标志物的全身表达情况。
这是一项针对新生血管性AMD患者和年龄匹配的健康对照个体的前瞻性病例对照研究。患者从门诊项目中招募，对照个体从患者亲属中招募。不包括当前吸烟者以及患有活动性免疫疾病或正在患癌症的个体，因为已知这些因素会影响中性粒细胞功能。采集新鲜抽取的静脉血进行中性粒细胞标志物的流式细胞术分析。我们确定阳性细胞百分比，并使用荧光强度测量比较表达水平。我们发现新生血管性AMD患者（n = 29）和对照组（n = 28）在标志物表达上存在条件差异：总体观察时未发现差异，但关注非吸烟者时出现了一些差异。在此，新生血管性AMD患者的活性标志物分化簇（CD）66b表达增加（P = 0.003；曼-惠特尼U检验），黏附标志物CD162表达降低（P = 0.044；曼-惠特尼U检验），炎症消退标志物C-X-C趋化因子受体2表达较低（P = 0.044；曼-惠特尼U检验）。
我们提供了新的证据表明，对吸烟敏感的循环中性粒细胞活性在新生血管性AMD患者中可能有所不同。
