Department of Obstetrics, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
Eur Rev Med Pharmacol Sci. 2017 Jul;21(14):3164-3168.
To investigate changes in the peripheral blood mRNA levels of oxytocin receptor (OXTR) and Zinc finger E-box-binding homeobox 1 (ZEB1) before and after progesterone dosing in pregnant women with threatened premature labor.
Blood samples were collected from 30 healthy pregnant women with 28- to 33+6-week gestational age and singleton pregnancy (group A) and from 30 pregnant women with singleton pregnancy and threatened premature labor before and 48 hours after progesterone dosing (groups B and C, respectively) for quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) assay to assess the OXTR and ZEB1 mRNA levels.
The OXTR mRNA level was higher in the group B than in the groups A and C, and the ZEB1 mRNA level was lower in the group B than in the groups A and C. Notably, no significant difference was found in the mRNA level of OXTR or ZEB1 between group A and group C.
The peripheral blood mRNA level of OXTR was increased, and that of ZEB1 was decreased in pregnant women with threatened premature labor. Progesterone helped to maintain pregnancy by readjusting the mRNA levels of OXTR and ZEB1.
探讨孕激素治疗先兆早产孕妇前后外周血催产素受体（OXTR）和锌指 E 盒结合同源框 1（ZEB1）mRNA 水平的变化。
采集 30 例 28-33+6 周单胎妊娠健康孕妇（A 组）和 30 例单胎妊娠先兆早产孕妇（B、C 组）的外周血，采用实时荧光定量逆转录聚合酶链反应（qRT-PCR）检测 OXTR 和 ZEB1mRNA 水平。
B 组外周血 OXTRmRNA 水平高于 A、C 组，ZEB1mRNA 水平低于 A、C 组；A、C 组 OXTR 或 ZEB1mRNA 水平无显著差异。
先兆早产孕妇外周血 OXTRmRNA 水平升高，ZEB1mRNA 水平降低。孕激素可能通过调节 OXTR 和 ZEB1mRNA 水平来维持妊娠。
