牛磺酸对干眼症模型的抗氧化和渗透保护作用。

Antioxidant and Osmoprotecting Activity of Taurine in Dry Eye Models.

机构信息

1 Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania , Catania, Italy .

2 Center for Research in Ocular Pharmacology-CERFO, University of Catania , Catania, Italy .

出版信息

J Ocul Pharmacol Ther. 2018 Jan/Feb;34(1-2):188-194. doi: 10.1089/jop.2017.0008. Epub 2017 Aug 3.

DOI:10.1089/jop.2017.0008

PMID:28771380

Abstract

PURPOSE

To assess the protective effects of ophthalmic formulations based on taurine (TAU) and sodium hyaluronate (SH) in ocular surface.

METHODS

Rabbit corneal epithelial cells [Statens Seruminstitut Rabbit Cornea (SIRC)] were subjected to oxidative stress (1 mM HO) and treated with the following formulations: 0.2% SH, 0.4% SH, 0.4% SH +0.5% TAU. Reactive oxygen species (ROS) were evaluated by commercial kit. Dry eye was induced by atropine sulfate and topical treatment was carried out with the following formulations: 0.2% SH, 0.4% SH, 0.4% SH +0.5% TAU. Schirmer's test, tear breakup time (TBUT), and tear osmolarity were evaluated. Furthermore, tear matrix metalloproteinase 9 (MMP-9) expression was assessed by Western blot.

RESULTS

TAU significantly (P < 0.05) decreased ROS production in SIRC after oxidative stress. Topical administration of atropine in the rabbit eye significantly (P < 0.05) reduced tear volume and TBUT. Tear osmolarity was also significantly (P < 0.05) modified by atropine treatment. All the altered parameters were significantly (P < 0.05) reversed by 0.5% TAU +0.4% SH treatment; furthermore, this formulation was more effective than SH alone. Moreover, tear levels of MMP-9 were significantly (P < 0.05) lower in the group treated with 0.5% TAU +0.4% SH.

CONCLUSIONS

Altogether these data suggest that TAU has a relevant antioxidant effect in corneal epithelial cells and prevents the ocular surface damage elicited by atropine. Therefore, our findings suggest that TAU in combination with SH may be useful in clinical practice to manage ocular surface diseases.

摘要

目的

评估牛磺酸（TAU）和透明质酸钠（SH）眼部制剂对眼表的保护作用。

方法

将兔角膜上皮细胞[Statens Seruminstitut Rabbit Cornea（SIRC）]置于氧化应激（1mM HO）下，并分别用以下制剂处理：0.2%SH、0.4%SH、0.4%SH+0.5%TAU。通过商业试剂盒评估活性氧（ROS）。用硫酸阿托品诱导干眼症，并使用以下制剂进行局部治疗：0.2%SH、0.4%SH、0.4%SH+0.5%TAU。评估泪液分泌试验（Schirmer 试验）、泪膜破裂时间（TBUT）和泪液渗透压。此外，通过 Western blot 评估泪液基质金属蛋白酶 9（MMP-9）的表达。

结果

TAU 可显著（P<0.05）降低氧化应激后 SIRC 中 ROS 的产生。阿托品滴眼可显著（P<0.05）减少兔眼的泪液量和 TBUT。泪液渗透压也因阿托品处理而显著（P<0.05）改变。所有改变的参数均经 0.5%TAU+0.4%SH 治疗显著（P<0.05）逆转；此外，该制剂比单独使用 SH 更有效。此外，经 0.5%TAU+0.4%SH 治疗组的泪液 MMP-9 水平显著（P<0.05）降低。