Salazar-Villatoro Lizbeth, Chávez-Munguía Bibiana, Guevara-Estrada Celia Esther, Lagunes-Guillén Anel, Hernández-Martínez Dolores, Castelan-Ramírez Ismael, Omaña-Molina Maritza
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Ciudad de Mexico 07360, Mexico.
Laboratorio de Amibas Anfizóicas, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, Mexico.
Pathogens. 2023 Aug 16;12(8):1049. doi: 10.3390/pathogens12081049.
spp. is the etiological agent of amoebic keratitis. In this study, the effect of taurine in physiological concentrations in tears (195 μM) on trophozoites of through the ex vivo amoebic keratitis model was evaluated. Trophozoites were coincubated with the Syrian golden hamster cornea () for 3 and 6 h. Group 1: Control (-). Corneas coincubated with amoebic culture medium and taurine. Group 2: Control (+). Corneas coincubated with trophozoites without taurine. Group 3: Corneas coincubated with taurine 15 min before adding trophozoites. Group 4: Trophozoites coincubated 15 min with taurine before placing them on the cornea. Group 5: Corneas coincubated for 15 min with trophozoites; subsequently, taurine was added. Results are similar for both times, as evaluated by scanning electron microscopy. As expected, in the corneas of Group 1, no alterations were observed in the corneal epithelium. In the corneas of Group 2, few adhered trophozoites were observed on the corneal surface initiating migrations through cell junctions as previously described; however, in corneas of Groups 3, 4 and 5, abundant trophozoites were observed, penetrating through different corneal cell areas, emitting food cups and destabilizing corneal surface in areas far from cell junctions. Significant differences were confirmed in trophozoites adherence coincubated with taurine ( < 0.05). Taurine does not prevent the adhesion and invasion of the amoebae, nor does it favor its detachment once these have adhered to the cornea, suggesting that taurine in the physiological concentrations found in tears stimulates pathogenic mechanisms of .
某属物种是阿米巴性角膜炎的病原体。在本研究中，通过体外阿米巴性角膜炎模型评估了泪液中生理浓度（195 μM）的牛磺酸对某属滋养体的影响。滋养体与叙利亚金黄地鼠角膜共同孵育3小时和6小时。第1组：对照（-）。角膜与阿米巴培养基和牛磺酸共同孵育。第2组：对照（+）。角膜与不含牛磺酸的滋养体共同孵育。第3组：在添加滋养体前15分钟将角膜与牛磺酸共同孵育。第4组：滋养体在放置于角膜上之前与牛磺酸共同孵育15分钟。第5组：角膜与滋养体共同孵育15分钟；随后添加牛磺酸。通过扫描电子显微镜评估，两个时间点的结果相似。如预期的那样，在第1组的角膜中，未观察到角膜上皮有改变。在第2组的角膜中，在角膜表面观察到少量附着的滋养体开始通过细胞连接迁移，如先前所述；然而，在第3、4和5组的角膜中，观察到大量滋养体，穿透不同的角膜细胞区域，发出食物杯并使远离细胞连接的区域的角膜表面不稳定。与牛磺酸共同孵育的滋养体附着情况有显著差异（P<0.05）。牛磺酸不能阻止阿米巴的粘附和侵袭，一旦它们粘附到角膜上，也不促进其脱离，这表明泪液中发现的生理浓度的牛磺酸会刺激某属的致病机制。
