Cai Yicun, He Yuping, Lv Rong, Chen Hongchao, Wang Qiang, Pan Liangwen
Technical Center for Animal, Plant and Food Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of China, Pudong New Area, Shanghai, China.
PLoS One. 2017 Aug 3;12(8):e0181949. doi: 10.1371/journal.pone.0181949. eCollection 2017.
Meat products often consist of meat from multiple animal species, and inaccurate food product adulteration and mislabeling can negatively affect consumers. Therefore, a cost-effective and reliable method for identification and quantification of animal species in meat products is required. In this study, we developed a duplex droplet digital PCR (dddPCR) detection and quantification system to simultaneously identify and quantify the source of meat in samples containing a mixture of beef (Bos taurus) and pork (Sus scrofa) in a single digital PCR reaction tube. Mixed meat samples of known composition were used to test the accuracy and applicability of this method. The limit of detection (LOD) and the limit of quantification (LOQ) of this detection and quantification system were also identified. We conclude that our dddPCR detection and quantification system is suitable for quality control and routine analyses of meat products.
肉类产品通常由多种动物的肉组成，食品掺假和标签错误会对消费者产生负面影响。因此，需要一种经济高效且可靠的方法来鉴定和定量肉类产品中的动物种类。在本研究中，我们开发了一种双重液滴数字PCR（dddPCR）检测和定量系统，可在单个数字PCR反应管中同时鉴定和定量含有牛肉（Bos taurus）和猪肉（Sus scrofa）混合物的样品中的肉源。使用已知成分的混合肉样品来测试该方法的准确性和适用性。还确定了该检测和定量系统的检测限（LOD）和定量限（LOQ）。我们得出结论，我们的dddPCR检测和定量系统适用于肉类产品的质量控制和常规分析。
