利用常规和实时 PCR 技术检测二进制肉混合物和一些商业食品中的猪肉。
Pork detection in binary meat mixtures and some commercial food products using conventional and real-time PCR techniques.
机构信息
Food Science & Nutrition Dept., College of Food & Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
Food Science & Nutrition Dept., College of Food & Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia; Department of Food Science, Faculty of Agriculture, Benha University, Benha 13518, Egypt.
出版信息
Food Chem. 2017 Mar 15;219:54-60. doi: 10.1016/j.foodchem.2016.09.108. Epub 2016 Sep 17.
Pork DNA was detected in meat mixtures using both conventional PCR and real-time PCR (RT-PCR). Thirty meat mixtures containing beef, chicken, camel, rabbit, goat and sheep with varying percentage of pork (0%, 1%, 5%, 10%, and 20%) and 75 commercial food products, were analyzed using conventional and RT-PCR to determine the presence of pork DNA. Pork DNA standard curves and cycle threshold (Ct) values were used for quantification. The detection limits for pork DNA in the mixtures were 0.22, 0.047, 0.048, 0.0000037, 0.015ng/μl respectively. Unlike conventional PCR, RT-PCR detected pork DNA in nine processed food samples [chicken sausages (2), chicken luncheon (2), turkey meat loaf, milk chocolate with soft nougat, jelly, cake, and candies] at pork DNA concentrations of 0.0001ng/μl or less.
使用常规 PCR 和实时 PCR（RT-PCR）在肉混合物中检测猪肉 DNA。分析了 30 种含有牛肉、鸡肉、骆驼肉、兔肉、羊肉和绵羊肉的肉混合物，其猪肉含量为 0%、1%、5%、10%和 20%，以及 75 种商业食品，使用常规 PCR 和 RT-PCR 来确定猪肉 DNA 的存在。使用猪肉 DNA 标准曲线和循环阈值 (Ct) 值进行定量。混合物中猪肉 DNA 的检测限分别为 0.22、0.047、0.048、0.0000037 和 0.015ng/μl。与常规 PCR 不同，RT-PCR 在 9 种加工食品样品[鸡肉香肠（2）、鸡肉午餐肉（2）、火鸡肉馅饼、牛奶巧克力软牛轧糖、果冻、蛋糕和糖果]中检测到猪肉 DNA，浓度为 0.0001ng/μl 或更低。
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