Department of Plant Biology and Soil Science, University of Vigo, Campus Lagoas-Marcosende s/n, E-36310 Vigo, Spain.
Department of Life Sciences and Systems Biology, University of Turin, Via Quarello 15/A, I-101335 Turin, Italy.
J Plant Physiol. 2017 Nov;218:56-65. doi: 10.1016/j.jplph.2017.07.014. Epub 2017 Jul 22.
The plant phenolic compound (E)-chalcone has been previously found to induce noticeable seedling size reduction and progressive de-greening (bleaching) in shoots of Arabidopsis thaliana seedlings. In this work, we demonstrate that this progressive de-greening occurring on Arabidopsis shoots after (E)-chalcone treatment, is directly linked to early plasma membrane depolarization and dramatic effects on chloroplasts structure and function. Later effects in chalcone-treated seedlings included ROS accumulation, pigment degradation, reduced photosynthetic activity, bleaching, and eventually cell death. De-greening and pigment degradation induced by (E)-chalcone were partially reversed when NaCl was added together with chalcone, which could be related to restoration of altered pH gradients. All these results suggest that rapid alteration of plasma membrane potential after chalcone treatment is a major component of the mode of action of (E)-chalcone on Arabidopsis metabolism.
植物类黄酮化合物（E）-查尔酮先前被发现可诱导拟南芥幼苗显著的苗大小减小和渐进的脱绿（漂白）。在这项工作中，我们证明了（E）-查尔酮处理后拟南芥茎上发生的这种渐进脱绿与早期质膜去极化以及对叶绿体结构和功能的巨大影响直接相关。在查尔酮处理的幼苗中，后期效应包括 ROS 积累、色素降解、光合作用活性降低、漂白，最终导致细胞死亡。当用查尔酮处理的同时添加 NaCl 时，（E）-查尔酮诱导的脱绿和色素降解部分得到逆转，这可能与恢复改变的 pH 梯度有关。所有这些结果表明，查尔酮处理后质膜电势的快速改变是（E）-查尔酮对拟南芥代谢作用模式的主要组成部分。
