Sánchez-Moreiras Adela M, Graña Elisa, Reigosa Manuel J, Araniti Fabrizio
Department of Plant Biology and Soil Science, Faculty of Biology, University of Vigo, Vigo, Spain.
CITACA, Agri-Food Research and Transfer Cluster, University of Vigo, Ourense, Spain.
Front Plant Sci. 2020 Dec 21;11:583590. doi: 10.3389/fpls.2020.583590. eCollection 2020.
Imaging of chlorophyll fluorescence (CFI) represents an easy, precise, fast and non-invasive technique that can be successfully used for discriminating plant response to phytotoxic stress with reproducible results and without damaging the plants. The spatio-temporal analyses of the fluorescence images can give information about damage evolution, secondary effects and plant defense response. In the last years, some studies about plant natural compounds-induced phytotoxicity have introduced imaging techniques to measure fluorescence, although the analysis of the image as a whole is often missed. In this paper we, therefore, evaluated the advantages of monitoring fluorescence images, presenting the physiological interpretation of different possible combinations of the most relevant parameters linked to fluorescence emission and the images obtained.
叶绿素荧光成像（CFI）是一种简单、精确、快速且非侵入性的技术，可成功用于区分植物对植物毒性胁迫的反应，结果具有可重复性且不会损害植物。荧光图像的时空分析可以提供有关损伤演变、次生效应和植物防御反应的信息。在过去几年中，一些关于植物天然化合物诱导的植物毒性的研究引入了成像技术来测量荧光，尽管对整个图像的分析常常被忽略。因此，在本文中，我们评估了监测荧光图像的优势，阐述了与荧光发射相关的最相关参数的不同可能组合以及所获得图像的生理学解释。
