Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630004, Tamil Nadu, India.
Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630004, Tamil Nadu, India.
J Photochem Photobiol B. 2017 Sep;174:133-143. doi: 10.1016/j.jphotobiol.2017.07.026. Epub 2017 Jul 25.
The control of Zika virus mosquito vectors and well as the development of drugs in the fight against biofilm-forming microbial pathogens, are timely and important challenges in current bionanoscience. Here we focused on the eco-friendly fabrication of Ag nanostructures using the seed extract of Pedalium murex, an ancient Indian medicinal plant. Initial confirmation of Ag nanoparticles (AgNPs) production was showed by a color change from transparent to dark brown. The UV-Visible spectrum (476nm), X-ray diffraction peaks (101, 200, 220 and 311) and Fourier transform infrared spectroscopy shed light on the production of green-capped AgNPs. Morphological structure analysis using HR-TEM showed that the AgNPs were mostly hexagonal in shape with rough edges, and a size of 20-30nm. The larvicidal potential of P. murex seed extract and AgNPs fabricated using the P. murex seed extract (Pm-AgNPs) was tested on fourth instar mosquito larvae of the Zika virus vector Aedes aegypti. Maximum efficacy was achieved by Pm-AgNPs against Ae. aegypti after 24h (LC 34.88; LC 64.56mg/ml), if compared to the P. murex seed extract. Histopathological analyses showed severe damages to the hindgut and larval muscles in NPs-treated Ae. aegypti larvae. The sub-MIC concentrations of Pm-AgNPs exhibited significant anti-biofilm activity against Gram positive (Enterococcus faecalis, Staphylococcus aureus) and Gram negative (Shigella sonnei, Pseudomonas aeruginosa) bacterial pathogens, as showed by EPS and MTP assays. Light and CLSM microscopic studies highlighted a significant impact of P. murex seed extract and Pm-synthesized AgNPs on the surface topography and architecture of bacterial biofilm, both in Gram positive and Gram negative species. Overall, results reported here contribute to the development of reliable large-scale protocols for the green fabrication of effective mosquito larvicides and biofilm inhibitors.
控制寨卡病毒蚊媒以及开发针对生物膜形成微生物病原体的药物，是当前生物纳米科学中及时且重要的挑战。在这里，我们专注于使用 Pedalium murex 的种子提取物来环保地制造 Ag 纳米结构，这是一种古老的印度药用植物。Ag 纳米粒子（AgNPs）生产的初步确认是通过从透明变为深棕色的颜色变化来显示的。紫外-可见光谱（476nm）、X 射线衍射峰（101、200、220 和 311）和傅里叶变换红外光谱表明生产了绿色帽 AgNPs。使用高分辨率透射电子显微镜（HR-TEM）进行的形态结构分析表明，AgNPs 大多呈六边形，具有粗糙的边缘，尺寸为 20-30nm。使用 P. murex 种子提取物制造的 P. murex 种子提取物和 AgNPs（Pm-AgNPs）对寨卡病毒载体埃及伊蚊的第四龄幼虫进行了杀幼虫测试。与 P. murex 种子提取物相比，Pm-AgNPs 对埃及伊蚊的最高功效在 24 小时后达到（LC 34.88；LC 64.56mg/ml）。组织病理学分析显示， NPs 处理的埃及伊蚊幼虫的后肠和幼虫肌肉受到严重损伤。Pm-AgNPs 的亚 MIC 浓度对革兰氏阳性（粪肠球菌、金黄色葡萄球菌）和革兰氏阴性（宋内志贺菌、铜绿假单胞菌）细菌病原体表现出显著的抗生物膜活性，如 EPS 和 MTP 测定所示。光和 CLSM 显微镜研究突出显示了 P. murex 种子提取物和 Pm 合成的 AgNPs 对革兰氏阳性和革兰氏阴性物种的细菌生物膜表面形貌和结构的重大影响。总的来说，这里报告的结果有助于开发可靠的大规模协议，用于绿色制造有效的杀蚊幼虫剂和生物膜抑制剂。
