Granada-Moreno C I, Aburto-Medina A, de Los Cobos Vasconcelos D, González-Sánchez A
Coordinación de Ingeniería Ambiental, Instituto de Ingeniería UNAM, Circuito Escolar, Ciudad Universitaria, Mexico City, Mexico.
Centre for Environmental Sustainability and Remediation, School of Applied Sciences, RMIT University, Bundoora, Vic., Australia.
J Appl Microbiol. 2017 Oct;123(4):903-915. doi: 10.1111/jam.13552. Epub 2017 Sep 12.
To achieve the functional specialization of a microalgae community through operational tuning of an open photobioreactor used for biogas upgrading under alkaline conditions.
An open photobioreactor was inoculated with an indigenous microalgae sample from the Texcoco Soda Lake. A microalgae community was adapted to fix CO from synthetic biogas through different culture conditions reaching a maximum of 220 mg CO l per day. Picochlorum sp. and Scenedesmus sp. were identified as the prominent microalgae genera by molecular fingerprinting (partial sequencing of 16S rRNA and 18S rRNA genes) but only the first was detected by microscopy screening. Changes in the microalgae community profile were monitored by a range-weighted richness index, reaching the lowest value when biogas was upgraded.
A robust microalgae community in the open photobioreactor was obtained after different culture conditions. The specialization of microalgae community for CO fixation under H S presence was driven by biogas upgrading conditions.
The alkaline conditions enhance the CO absorption from biogas and could optimize specialized microalgae communities in the open photobioreactor. Denaturing gradient gel electrophoresis fingerprinting and richness index comparison are useful methods for the evaluation of microalgae community shifts and photosynthetic activity performance, particularly in systems intended for CO removal from biogas where the CO assimilation potential can be related to the microbial richness.
通过对用于碱性条件下沼气升级的开放式光生物反应器进行操作调整，实现微藻群落的功能专业化。
用来自特斯科科盐湖的本地微藻样本接种一个开放式光生物反应器。通过不同的培养条件使微藻群落适应从合成沼气中固定二氧化碳，最高达到每天220毫克二氧化碳/升。通过分子指纹图谱（16S rRNA和18S rRNA基因的部分测序）鉴定出皮氏藻属和栅藻属为主要的微藻属，但通过显微镜筛选仅检测到了前者。通过范围加权丰富度指数监测微藻群落谱的变化，在沼气升级时达到最低值。
经过不同培养条件后，在开放式光生物反应器中获得了一个稳健的微藻群落。在硫化氢存在下，微藻群落对二氧化碳固定的专业化是由沼气升级条件驱动的。
碱性条件增强了沼气中二氧化碳的吸收，并可优化开放式光生物反应器中的专业化微藻群落。变性梯度凝胶电泳指纹图谱和丰富度指数比较是评估微藻群落变化和光合活性性能的有用方法，特别是在旨在从沼气中去除二氧化碳的系统中，其中二氧化碳同化潜力可能与微生物丰富度相关。
