Photorespiration in an outdoor alkaline open-photobioreactor used for biogas upgrading.

The rates of oxygenic and carbon fixation photosynthetic processes of a microalgae consortium were simultaneously evaluated under steady-state performance in an bench scale alkaline open-system exposed to outdoor conditions in Mexico City. A synthetic methane-free gaseous stream (SMGS) similar to biogas was used as inorganic carbon source and model of biogas upgrading. The microalgae CO fixation rates were calculated through a novel methodology based on an inorganic carbon mass balance under continuous scrubbing of a SMGS similar to biogas, where the influence of pH and temperature time-depended oscillations were successfully incorporated into the mass balances. The oxygenic activity and carbon fixation occurred at different non-stoichiometric rates during the diurnal phase, in average carbon fixation predominated over oxygen production (photosynthesis quotient PQ≈ 0.5 mol O mol CO) indicating photorespiration occurrence mainly under dissolved oxygen concentrations higher than 10 mg L. The oxygen and inorganic carbon mass balances demonstrated that photorespiration and endogenous respiration were responsible for losing up to 66% and 7% respectively of the biomass grew at diurnal periods under optimal conditions. In favoring photorespiration conditions, the microalgae biomass productivity (CO effectively captured) can be severely decreased. A kinetic mathematical model as a function of temperature and irradiance of the oxygenic photosynthetic activity indicated the optimal operation zone for this outdoor alkaline open-photobioreactor, where irradiance was found being the most influential parameter.