Liu Lin, Sun Ting, Ren Huizhu
Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
Materials (Basel). 2017 Jan 5;10(1):40. doi: 10.3390/ma10010040.
Metal nanoparticles (NPs) possess unique physicochemical attributes for creating effective recognition and transduction processes in chem/bio-sensing. In this work, we suggested that citrate-capped Au/Ag NPs could be used as the reporters for the design of hydrogen peroxide (H₂O₂) sensors with a simple manipulation principle and an easy detection procedure. Specifically, -benzenediboronic acid (BDBA) induced the aggregation of citrate-capped Au NPs through the cross-linking reaction between citrate and boronic acid of BDBA in solution. By modifying the electrode with a boronic acid derivative, the BDBA-induced assembly of Au NPs was achieved on the electrode surface. This led to a significant decrease in the electron transfer resistance due to the unique conductive ability of Au NPs. However, when the boronate group on the electrode surface was oxidized into its phenol format, the assembly of Au NPs on the electrode surface was not achieved. As a result, a higher electron transfer resistance was observed. The process could be monitored by electrochemical impedance technique. Furthermore, when Ag NPs were used instead of Au NPs in this design, the H₂O₂ concentration could be determined by measuring the linear-sweep voltammetry (LSV) current through the solid-state Ag/AgCl reaction of Ag NPs. The results indicated that NP-based colorimetric assays could be developed into more sensitive electrochemical analysis.
金属纳米颗粒（NPs）具有独特的物理化学特性，可用于在化学/生物传感中创建有效的识别和转导过程。在这项工作中，我们提出柠檬酸盐包覆的金/银纳米颗粒可以用作设计过氧化氢（H₂O₂）传感器的报告分子，其操作原理简单，检测程序容易。具体而言，对苯二硼酸（BDBA）通过溶液中柠檬酸盐与BDBA硼酸之间的交联反应诱导柠檬酸盐包覆的金纳米颗粒聚集。通过用硼酸衍生物修饰电极，在电极表面实现了BDBA诱导的金纳米颗粒组装。由于金纳米颗粒独特的导电能力，这导致电子转移电阻显著降低。然而，当电极表面的硼酸酯基团氧化成其酚形式时，在电极表面未实现金纳米颗粒的组装。结果，观察到更高的电子转移电阻。该过程可以通过电化学阻抗技术进行监测。此外，当在该设计中使用银纳米颗粒代替金纳米颗粒时，可以通过测量银纳米颗粒的固态Ag/AgCl反应的线性扫描伏安法（LSV）电流来确定H₂O₂浓度。结果表明，基于纳米颗粒的比色测定法可以发展成为更灵敏的电化学分析方法。
