Zhang Jinlong, Mai Bixia, Cai Tingwei, Luo Jiayi, Wu Wanhan, Liu Bing, Han Ningxu, Xing Feng, Deng Xu
Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Guangdong Province Key Laboratory of Durability for Marine Civil Engineering, The Key Laboratory on Durability of Civil Engineering in Shenzhen, School of Civil Engineering, Shenzhen University, Shenzhen 518060, China.
Materials (Basel). 2017 Jan 26;10(2):116. doi: 10.3390/ma10020116.
An optimized strategy for the enhancement of microbially induced calcium precipitation including spore viability ensurance, nutrient selection and O2 supply was developed. Firstly, an optimal yeast extract concentration of 5 g/l in sporulation medium was determined based on viable spore yield and spore viability. Furthermore, the effects of certain influential factors on microbial calcium precipitation process of H4 in the presence of oxygen releasing tablet (ORT) were evaluated. The results showed that CaO2 is preferable to other peroxides in improving the calcium precipitation by H4. H4 strain is able to precipitate a highly insoluble calcium at the CaO2 dosage range of 7.5-12.5 g/l, and the most suitable spore concentration is 6 × 108 spores/ml when the spore viability (viable spore ratio) is approximately 50%. Lactate is the best carbon source and nitrate is the best nitrogen source for aerobic incubation. This work has laid a foundation of ternary self-healing system containing bacteria, ORT, and nutrients, which will be promising for the self-healing of cracks deep inside the concrete structure.
开发了一种优化策略来增强微生物诱导的钙沉淀，包括确保孢子活力、选择营养物质和供应氧气。首先，根据活孢子产量和孢子活力，确定了孢子形成培养基中酵母提取物的最佳浓度为5 g/l。此外，评估了某些影响因素对在释氧片（ORT）存在下H4微生物钙沉淀过程的影响。结果表明，在改善H4的钙沉淀方面，CaO2比其他过氧化物更具优势。H4菌株在7.5-12.5 g/l的CaO2剂量范围内能够沉淀出高度不溶性的钙，当孢子活力（活孢子比率）约为50%时，最合适的孢子浓度为6×108个孢子/ml。乳酸是需氧培养的最佳碳源，硝酸盐是最佳氮源。这项工作为包含细菌、ORT和营养物质的三元自修复系统奠定了基础，这对于混凝土结构内部深处裂缝的自修复具有广阔前景。
